rdf:type |
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lifeskim:mentions |
umls-concept:C0001734,
umls-concept:C0017817,
umls-concept:C0018557,
umls-concept:C0022646,
umls-concept:C0023884,
umls-concept:C0024554,
umls-concept:C0034693,
umls-concept:C0054252,
umls-concept:C0441889,
umls-concept:C1167622,
umls-concept:C1280500
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pubmed:issue |
1
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pubmed:dateCreated |
1994-4-12
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pubmed:abstractText |
The effects of pretreatment of buthionine sulfoximine (BSO) alone or in combination with diethylmaleate (DEM) on glutathione (GSH) levels and aflatoxin B1 (AFB1)-DNA binding have been examined in livers and kidneys of young male Fischer rats and Syrian golden hamsters 2 h after an intraperitoneal injection of [3H]AFB1 (0.4 mg/kg body wt.). Animals were treated with BSO (4 mmol/kg body wt.) alone at 4 h and 2 h or with DEM (3 mmol/kg body wt.) at 4 h and BSO at 2 h before AFB1 injection. Hepatic AFB1-DNA binding was about 29.0 and 6.0 pmol/mg DNA in rats and hamsters, respectively. In rats, BSO increased AFB1-DNA binding by about 40% with a drop in GSH by 70%. Treatment with DEM-BSO increased AFB1-DNA binding by about 80% with a concomitant drop in GSH in both species. In hamsters, BSO increased AFB1-DNA binding by only 10% with a 50% drop in GSH. The kidneys of both species have lower GSH levels and AFB1-DNA binding than their respective liver tissues. The effect of BSO alone or in combination with DEM on both GSH levels and AFB1-DNA binding are comparable even though BSO alone is less effective in both species. The role of modulation of GSH levels on AFB1-DNA binding and hence biological effects of AFB1 in these two species is discussed.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aflatoxin B1,
http://linkedlifedata.com/resource/pubmed/chemical/Antimetabolites, Antineoplastic,
http://linkedlifedata.com/resource/pubmed/chemical/Buthionine Sulfoximine,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Adducts,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione,
http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Transferase,
http://linkedlifedata.com/resource/pubmed/chemical/Maleates,
http://linkedlifedata.com/resource/pubmed/chemical/Methionine Sulfoximine,
http://linkedlifedata.com/resource/pubmed/chemical/aflatoxin B1-DNA adduct,
http://linkedlifedata.com/resource/pubmed/chemical/diethyl maleate
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0304-3835
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
76
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
25-30
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8124663-Aflatoxin B1,
pubmed-meshheading:8124663-Animals,
pubmed-meshheading:8124663-Antimetabolites, Antineoplastic,
pubmed-meshheading:8124663-Biotransformation,
pubmed-meshheading:8124663-Buthionine Sulfoximine,
pubmed-meshheading:8124663-Cricetinae,
pubmed-meshheading:8124663-DNA,
pubmed-meshheading:8124663-DNA Adducts,
pubmed-meshheading:8124663-Glutathione,
pubmed-meshheading:8124663-Glutathione Transferase,
pubmed-meshheading:8124663-Kidney,
pubmed-meshheading:8124663-Liver,
pubmed-meshheading:8124663-Male,
pubmed-meshheading:8124663-Maleates,
pubmed-meshheading:8124663-Mesocricetus,
pubmed-meshheading:8124663-Methionine Sulfoximine,
pubmed-meshheading:8124663-Rats,
pubmed-meshheading:8124663-Rats, Inbred F344,
pubmed-meshheading:8124663-Time Factors
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pubmed:year |
1994
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pubmed:articleTitle |
Effect of glutathione levels on aflatoxin B1-DNA binding in livers and kidneys of male rats and hamsters pretreated with buthionine sulfoximine and dimethylmaleate.
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pubmed:affiliation |
Fels Institute for Cancer Research and Molecular Biology, Temple University School of Medicine, Philadelphia, PA 19140.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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