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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1994-4-14
|
| pubmed:abstractText |
Acute or chronic exposure to cadmium (Cd) causes severe organ damages with the infiltration of leukocytes, neutrophils in particular occurring in the acute phase. Interleukin-8 (IL-8), a novel neutrophil chemotactic and activating cytokine, is produced by various types of cells in response to a wide variety of inflammatory stimuli. The administration of an antibody against IL-8 has been shown to inhibit neutrophil infiltration in several animal models, indicating a causal relationship between IL-8 and neutrophil infiltration. Hence, in this study we investigated whether Cd induced IL-8 production in human peripheral blood mononuclear cells (PBMC). Cd, over a wide range of concentrations, did induce human PBMC to produce large amounts of bioactive IL-8, the maximal induction being observed at 10(-4) M. The production was inhibited specifically by a metal chelating agent, ethylenediaminetetraacetic acid (EDTA). Steady level of IL-8 mRNA increased within 30 min after the addition of Cd and reached a maximal level at 2 h, decreasing thereafter. A protein synthesis inhibitor, cycloheximide, failed to inhibit IL-8 mRNA accumulation, indicating that new protein synthesis was not required for IL-8 mRNA induction. Concomitantly with the induction of IL-8, within 10 min Cd generated reactive oxygen intermediates (ROI) in human PBMC. A radical scavenger, N-acetyl-L-cysteine (NAC), inhibited both IL-8 production and the generation of ROI, implying the possible involvement of ROI in IL-8 production. This notion was also supported by our findings that a superoxide generating agent, paraquat, induced IL-8 production in human PBMC and that NAC blocked this paraquat-induced IL-8 production.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cadmium,
http://linkedlifedata.com/resource/pubmed/chemical/Cycloheximide,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-8,
http://linkedlifedata.com/resource/pubmed/chemical/L-Lactate Dehydrogenase,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Superoxides
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
1056-5477
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
12
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
421-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8123758-Base Sequence,
pubmed-meshheading:8123758-Blotting, Northern,
pubmed-meshheading:8123758-Cadmium,
pubmed-meshheading:8123758-Cells, Cultured,
pubmed-meshheading:8123758-Chemotaxis, Leukocyte,
pubmed-meshheading:8123758-Consensus Sequence,
pubmed-meshheading:8123758-Cycloheximide,
pubmed-meshheading:8123758-Dose-Response Relationship, Drug,
pubmed-meshheading:8123758-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:8123758-Gene Expression,
pubmed-meshheading:8123758-Humans,
pubmed-meshheading:8123758-Interleukin-8,
pubmed-meshheading:8123758-Kinetics,
pubmed-meshheading:8123758-L-Lactate Dehydrogenase,
pubmed-meshheading:8123758-Leukocytes, Mononuclear,
pubmed-meshheading:8123758-Molecular Sequence Data,
pubmed-meshheading:8123758-RNA, Messenger,
pubmed-meshheading:8123758-Superoxides,
pubmed-meshheading:8123758-Time Factors
|
| pubmed:year |
1993
|
| pubmed:articleTitle |
Cadmium induces interleukin-8 production in human peripheral blood mononuclear cells with the concomitant generation of superoxide radicals.
|
| pubmed:affiliation |
Department of Pharmacology, Kanazawa University, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|