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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1994-4-7
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pubmed:abstractText |
arsR, the first gene of the Staphylococcus xylosus (pSX267) arsenic/antimonite resistance (ars) operon encodes a negative regulatory protein, ArsR, which mediates inducibility of the resistances by arsenic and antimony compounds. ArsR, which has no obvious DNA-binding motif in its primary structure, was purified from an ArsR-overproducing Escherichia coli strain and identified as a DNA-binding protein by its behaviour in gel mobility shift assays. ArsR had a specific affinity for a 312 bp DNA restriction fragment carrying the ars promoter; the minimum sequence complexed by ArsR was a 75 bp polymerase chain reaction (PCR) fragment, which mainly comprised the -35 and -10 regions of the promoter. The effect of inducers on the DNA-binding activity of ArsR was examined by in vitro induction assays; only arsenite inhibited DNA-binding of the repressor. DNase I footprinting revealed two protected regions within the promoter region, spanning 23 and 9 nucleotides, respectively. Furthermore, a new cleavage site for DNase I between the protected regions was made accessible by binding of the repressor. The footprints cover a region of three inverted repeats located between the -35 and -10 motifs of the ars promoter. By high resolution footprinting with the hydroxy radical, five sites of close contact between the protein and DNA were identified.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
|
pubmed:issn |
0026-8925
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
242
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
|
pubmed:pagination |
566-72
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8121414-Antimony,
pubmed-meshheading:8121414-Arsenic,
pubmed-meshheading:8121414-Base Sequence,
pubmed-meshheading:8121414-Drug Resistance, Microbial,
pubmed-meshheading:8121414-Gene Expression Regulation, Bacterial,
pubmed-meshheading:8121414-Molecular Sequence Data,
pubmed-meshheading:8121414-Operon,
pubmed-meshheading:8121414-Promoter Regions, Genetic,
pubmed-meshheading:8121414-Repressor Proteins,
pubmed-meshheading:8121414-Staphylococcus
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pubmed:year |
1994
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pubmed:articleTitle |
Binding of ArsR, the repressor of the Staphylococcus xylosus (pSX267) arsenic resistance operon to a sequence with dyad symmetry within the ars promoter.
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pubmed:affiliation |
Lehrstuhl für Mikrobielle Genetik, Universität Tübingen, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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