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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1994-4-6
|
| pubmed:abstractText |
Two B-cell lines, designated as HF-1 and HF-4, were characterised. The cell lines have complicated karyotype abnormalities including a 14;18 translocation and an 8q24 breakpoint originating from t(2;8)(p11;q24) (HF-1) or t(1;8)(p21;q24) (HF-4). The lines have BCL2 rearrangement and they are positive for CD19, CD20, CD22, CD39. HF-1 is also positive for IgG, and HF-4 is positive for IgM and IgD. On Northern blot analyses, the 2.6-kb and 4.2-kb transcripts corresponding to the major transcripts of CMYC and BCL2, respectively, were seen. In Western blot as well as in FACS (fluorescence-activated cell sorting) analysis the presence of BCL2 protein in the both HF-1 and HF-4 cells was demonstrated. The cell lines are expected to serve as an important tool in the study of the chromosomal mechanism activating cellular oncogenes, the somatic hypermutation mechanism of antigen-activated B cells and the apoptosis of B cells.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0902-4441
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
52
|
| pubmed:geneSymbol |
BCL2,
CMYC
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
65-72
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8119385-Base Sequence,
pubmed-meshheading:8119385-DNA Primers,
pubmed-meshheading:8119385-Gene Expression,
pubmed-meshheading:8119385-Gene Rearrangement,
pubmed-meshheading:8119385-Genes, Immunoglobulin,
pubmed-meshheading:8119385-Genes, myc,
pubmed-meshheading:8119385-Humans,
pubmed-meshheading:8119385-Immunophenotyping,
pubmed-meshheading:8119385-Karyotyping,
pubmed-meshheading:8119385-Lymph Nodes,
pubmed-meshheading:8119385-Lymphoma, B-Cell,
pubmed-meshheading:8119385-Molecular Sequence Data,
pubmed-meshheading:8119385-Oncogenes,
pubmed-meshheading:8119385-Proto-Oncogene Proteins,
pubmed-meshheading:8119385-Proto-Oncogene Proteins c-bcl-2,
pubmed-meshheading:8119385-RNA, Messenger,
pubmed-meshheading:8119385-Translocation, Genetic,
pubmed-meshheading:8119385-Tumor Cells, Cultured
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Two novel human B-cell lymphoma lines of lymphatic follicle origin: cytogenetic, molecular genetic and histopathological characterisation.
|
| pubmed:affiliation |
Department of Medical Genetics, University of Helsinki, Finland.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|