Linked Life Data

8118431

Source:http://linkedlifedata.com/resource/pubmed/id/8118431

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1994-4-4
pubmed:abstractText
Monoclonal antibodies against progesterone conjugated to carrier protein through substituent on the A-ring (C3 position ) or the C-ring (C11 position) of progesterone were used in the enzyme immunoassay. Antibody specificities were determined by testing the ability of 11 represensitive steroids to displace labelled progesterone in a competitive enzyme immuno-assay (EIA) and a radioimmunoassay (RIA). Immunization with progesterone conjugated to BSA through substituent on the A-ring (C3) resulted in the formation of monoclonal antibody (mAb) which fairly specific for progesterone. While immunization with progesterone at the 11-position (C11) resulted in the mAbs which were very specific for progesterone. The best EIA system was developed by using mAb against progesterone-11 alpha -hemisuccinyl BSA and tracer of progesterone-3(O-carboxymethyl oxmine)-horseradish peroxidase. Our approach is to confirm the overall orientation of the steroid in the binding site. Immunobiochemical analysis of mAb suggested that the D-ring is substantially more buried in the binding pocket than the A-ring. Our assay was designed to use the reagent inactivating cholesterol binding globulin in serum so that the extraction of the hormone into organic solvent was unnecessary. Therefore, our assay system can detect directly and rapidly the progesterone level in serum within one hour.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
1039-9712
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
553-63
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Influence of the conjugation site on the specificity of monoclonal antibodies to progesterone and on the performance of direct enzyme immunoassay.
pubmed:affiliation
Lab. of Immunochemistry, Korea Institute of Science & Technology (KIST), Japan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't