Linked Life Data

8112430

Source:http://linkedlifedata.com/resource/pubmed/id/8112430

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1994-3-31
pubmed:abstractText
A thymic stromal cell line with a medullary phenotype (Z210R.1) supported the differentiation of surface IgM+ B cells when cocultured with fetal liver cells in vitro. Conditioned medium (CM) from this cell line supported the long-term growth of a B cell line (NAG8/7) isolated from cocultures and enhanced the proliferation of unfractionated thymocytes to suboptimal concentrations of anti-CD3 antibodies in vitro. Biological assays of the CM detected interleukin-7 (IL-7) but not IL-1, IL-2, IL-3, IL-4, IL-6, Steel factor (SCF), leukemia inhibitory factor (LIF), or macrophage or granulocyte colony-stimulating factors (M-CSF or G-CSF). The failure of recombinant IL-7 to maintain the long-term growth of NAG8/7 cells and the inability of anti-IL-7 antibodies to significantly affect the response of either NAG8/7 cells or thymocytes to CM suggested the presence of one or more other cytokines in the CM. Analysis of concentrated CM fractionated by anion exchange chromatography revealed a single peak of activity in the NAG8/7 assay with an elution profile that was distinct from IL-7. Two peaks of activity were detected in the thymocyte response to anti-CD3 antibodies; one corresponded to IL-7 and the other corresponded to the same fractions that stimulated NAG8/7 cells. The second peak of thymocyte stimulatory activity could not be inhibited by neutralizing anti-IL-7 antibodies. In addition to producing a cytokine with unique properties, this thymic stromal cell exhibits a functional homology to bone marrow or fetal liver stromal cells not previously appreciated.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0301-472X
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
321-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8112430-Animals, pubmed-meshheading:8112430-Antibodies, Monoclonal, pubmed-meshheading:8112430-Antigens, CD3, pubmed-meshheading:8112430-B-Lymphocytes, pubmed-meshheading:8112430-Cell Communication, pubmed-meshheading:8112430-Cell Differentiation, pubmed-meshheading:8112430-Cell Line, pubmed-meshheading:8112430-Chromatography, Ion Exchange, pubmed-meshheading:8112430-Culture Media, Conditioned, pubmed-meshheading:8112430-Flow Cytometry, pubmed-meshheading:8112430-Growth Substances, pubmed-meshheading:8112430-Hematopoiesis, pubmed-meshheading:8112430-Immunoglobulin M, pubmed-meshheading:8112430-Interleukin-7, pubmed-meshheading:8112430-Liver, pubmed-meshheading:8112430-Mice, pubmed-meshheading:8112430-Mice, Inbred BALB C, pubmed-meshheading:8112430-Phenotype, pubmed-meshheading:8112430-Receptors, Antigen, B-Cell, pubmed-meshheading:8112430-T-Lymphocytes, pubmed-meshheading:8112430-Thymus Gland
pubmed:year
1994
pubmed:articleTitle
A thymic stromal cell line supports in vitro development of surface IgM+ B cells and produces a novel growth factor affecting B and T lineage cells.
pubmed:affiliation
Department of Immunology, University of Washington, Seattle 98195.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't