Linked Life Data

8110746

Source:http://linkedlifedata.com/resource/pubmed/id/8110746

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1994-3-28
pubmed:abstractText
The thermodynamics of binding of concanavalin A (Con A) with a series of linear and branched chain oligosaccharides including certain N-linked complex type and oligomannose type carbohydrates and a fraction of quail ovalbumin containing Man7 and Man8 oligomannose chains have been determined using titration microcalorimetry. Methyl3,6-di-O-(alpha-D-mannopyranosyl)-alpha-D-mannopyranoside, a branch chain trisaccharide moiety found in all N-linked carbohydrates which possesses approximately 60-fold higher affinity than methyl alpha-D-mannopyranoside, exhibited a change in enthalpy of binding (delta H) of -14.4 kcal mol-1 as compared to -8.2 kcal mol-1 for the monosaccharide. This demonstrates that Con A possesses an extended binding site for the trimannoside. However, a biantennary complex type carbohydrate with terminal beta (1,2)-GlcNAc residues which binds with 3-fold higher affinity than the trimannoside possesses a delta H of only -10.6 kcal mol-1. A plot of -delta H versus -T delta S for the carbohydrates in the present study showed positive deviations in -T delta S for the complex type carbohydrate, as well as alpha (1,2)-di- and trimannosyl oligosaccharides which are part of the structures of oligomannose type carbohydrates. The relative favorable changes in binding entropies of these compounds are attributed to the presence of multiple internal and terminal residues in each molecule which can independently bind to the monosaccharide binding site of the lectin. The delta H values for the complex type carbohydrate and the alpha (1,2) mannose oligosaccharides were also approximately -2.5 kcal mol-1 greater than that of methyl alpha-D-mannopyranoside, indicating some extended binding site interactions.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0006-2960
pubmed:author
pubmed:issnType
Print
pubmed:day
8
pubmed:volume
33
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1149-56
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Thermodynamics of lectin-carbohydrate interactions. Titration microcalorimetry measurements of the binding of N-linked carbohydrates and ovalbumin to concanavalin A.
pubmed:affiliation
Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York 10461.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.