Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1993-11-2
pubmed:abstractText
Salmonella enteritidis 27655-3b and a few diarrheagenic Escherichia coli strains produce morphologically and antigenically related, thin, aggregative fimbriae, collectively named GVVPQ fimbriae (S. K. Collinson, L. Emödy, T. J. Trust, and W. W. Kay, J. Bacteriol. 174:4490-4495, 1992). To determine whether GVVPQ fimbriae are common to Salmonella spp. and other enteropathogenic members of the family Enterobacteriaceae, 113 isolates were phenotypically screened for Congo red binding and aggregative colony morphology. Presumptive positive and representative negative strains were examined by Western blotting (immunoblotting) by using antiserum to SEF 17, the native GVVPQ fimbria of S. enteritidis. Only four S. enteritidis strains and six E. coli isolates possessed substantial amounts of GVVPQ fimbriae after 24 h of incubation on T medium. Following 5 days of incubation, 56 of 93 Salmonella isolates (60%) and 1 of 7 additional E. coli clinical isolates possessed detectable levels of GVVPQ fimbriae. Since variable expression of GVVPQ fimbriae was observed among Salmonella isolates and some E. coli strains produced scant amounts, as revealed by immunoelectron microscopy, the ability to produce these fimbriae was evaluated by genotypic screening. The structural gene for the SEF 17 fimbrin, agfA, was amplified by the polymerase chain reaction, cloned, and sequenced to provide a characterized DNA probe. An agfA DNA fragment hybridized strongly to 603 of 604 (99.8%) Salmonella isolates but very weakly to 31 of 266 other members of the family Enterobacteriaceae including 26 of 137 E. coli strains, 3 of 14 Citrobacter spp., and single isolates of Shigella sonnei and Enterobacter cloacae. The agfA DNA probe proved to be a valuable diagnostic tool for Salmonella isolates arrayed on hydrophobic grid membrane filters. Unique agfA sequences were targeted in the development of a polymerase chain reaction assay specific for Salmonella spp.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-1280609, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-1348439, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-1355852, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-1357528, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-1608468, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-1618717, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-1624441, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-1645840, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-1677356, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-1677357, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-1763061, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-1794363, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-1939595, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-1960105, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-1973367, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-1974320, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-2200698, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-2349239, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-2376546, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-2413068, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-2544889, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-2572604, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-2646710, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-2662196, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-2680975, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-2695752, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-2832831, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-2898203, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-2985470, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-2999794, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-3045151, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-3073312, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-3535069, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-3877720, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-6360046, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-6372975, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-8093237, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-8095317, http://linkedlifedata.com/resource/pubmed/commentcorrection/8104955-8098328
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
31
pubmed:geneSymbol
agfA
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2263-73
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
DNA-based diagnostic tests for Salmonella species targeting agfA, the structural gene for thin, aggregative fimbriae.
pubmed:affiliation
Department of Biochemistry and Microbiology, University of Victoria, British Columbia, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't