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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1993-10-26
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pubmed:abstractText |
The authors describe work in progress at the laboratory in Brescia, Italy, on the application of molecular methods to the diagnosis of leptospirosis. This work includes the following: a) Development of polymerase chain reaction (PCR) assays capable of amplifying specific deoxyribonucleic acid fragments from most Leptospira interrogans strains. b) Development of a microtitre-based assay for rapid detection of PCR-positive samples. c) Characterisation of Leptospira strains through restriction endonuclease analysis of PCR products and amplified fragment length polymorphism.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
|
pubmed:issn |
0253-1933
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
12
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
647-63
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8104548-Animals,
pubmed-meshheading:8104548-Animals, Domestic,
pubmed-meshheading:8104548-Antibodies, Bacterial,
pubmed-meshheading:8104548-Antigens, Bacterial,
pubmed-meshheading:8104548-DNA, Bacterial,
pubmed-meshheading:8104548-Italy,
pubmed-meshheading:8104548-Leptospira interrogans,
pubmed-meshheading:8104548-Leptospirosis,
pubmed-meshheading:8104548-Nucleic Acid Hybridization,
pubmed-meshheading:8104548-Polymerase Chain Reaction,
pubmed-meshheading:8104548-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:8104548-Restriction Mapping
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pubmed:year |
1993
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pubmed:articleTitle |
The search for improved methods for diagnosing leptospirosis: the approach of a laboratory in Brescia, Italy.
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pubmed:affiliation |
Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia, Brescia, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|