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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1993-7-30
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pubmed:abstractText |
The possible mechanism(s) responsible for the different effects exerted by proliferative stimuli of different nature on the appearance of enzyme-altered hepatic foci, were investigated in male Wistar rats. Rats given an initiating dose of diethylnitrosamine (150 mg/kg body weight) were fed a diet containing 0.03% acetylaminofluorene for 2 weeks. Between the first and the second week, cell proliferation was induced by a proliferative stimulus of compensatory type (partial hepatectomy) or by a direct mitogenic stimulus (lead nitrate, 100 mumol/kg). The effect of the two different proliferative stimuli on the appearance of gamma-glutamyl transferase-positive foci was monitored by killing the rats for examination at 1, 2, 3, 5, and 6 days after the induction of cell proliferation. The results indicate that while enzyme-altered hepatocytes can be observed as early as 3 days after partial hepatectomy and are characterized by a rapid growth, direct hyperplasia did not exert any effect on the growth capacity of initiated cells. No effect of lead nitrate-induced hyperplasia was observed following three administrations of the mitogen. When platelet-poor plasma taken from animals exposed to the different proliferative stimuli was tested in primary cultures of hepatocytes, it was found that it induced a significant increase in the labeling index of normal hepatocytes. However, while serum taken 6 days after partial hepatectomy was still able to induce a significant increase in the labeling index, platelet-poor plasma from lead-treated rats had lost part of its effect at 5 days after treatment. The inability of direct hyperplasia to stimulate the development of enzyme-altered hepatic foci was not unique to lead nitrate since the same phenomenon was observed when three other hepatomitogens, nafenopin, cyproterone acetate, and ethylene dibromide, were used.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-Acetylaminofluorene,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Diethylnitrosamine,
http://linkedlifedata.com/resource/pubmed/chemical/Lead,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogens,
http://linkedlifedata.com/resource/pubmed/chemical/Nitrates,
http://linkedlifedata.com/resource/pubmed/chemical/gamma-Glutamyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/lead nitrate
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0910-5050
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
84
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
501-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8100563-2-Acetylaminofluorene,
pubmed-meshheading:8100563-Animals,
pubmed-meshheading:8100563-Cells, Cultured,
pubmed-meshheading:8100563-DNA,
pubmed-meshheading:8100563-Diethylnitrosamine,
pubmed-meshheading:8100563-Hepatectomy,
pubmed-meshheading:8100563-Lead,
pubmed-meshheading:8100563-Liver,
pubmed-meshheading:8100563-Liver Neoplasms, Experimental,
pubmed-meshheading:8100563-Liver Regeneration,
pubmed-meshheading:8100563-Male,
pubmed-meshheading:8100563-Mitogens,
pubmed-meshheading:8100563-Nitrates,
pubmed-meshheading:8100563-Rats,
pubmed-meshheading:8100563-Rats, Wistar,
pubmed-meshheading:8100563-gamma-Glutamyltransferase
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pubmed:year |
1993
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pubmed:articleTitle |
Different effects of regenerative and direct mitogenic stimuli on the growth of initiated cells in the resistant hepatocyte model.
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pubmed:affiliation |
Istituto di Patologia Sperimentale, University of Cagliari, Italy.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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