Linked Life Data

8098609

Source:http://linkedlifedata.com/resource/pubmed/id/8098609

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1993-6-18
pubmed:abstractText
Nerve-muscle cocultures were used to study the relationship between neuronal Ca2+ level and the earliest nerve-muscle interaction. Xenopus spinal cord neurons were loaded with Ca2+ indicators and monitored by digital video microscopy as a myoball was manipulated into contact with it. Transmitter release was measured from the myoball by whole-cell recording. We observed a 1.5- to 6-fold increase in Ca2+ level in the neurite upon contact with a myoball. Fifty percent of the pairs showing Ca2+ elevation were also positive for neurotransmission. This Ca2+ rise was suppressed by lifting away the myoball, by Ca(2+)-free solution, or by suramin, a polyanionic compound that interferes with cell surface receptors. This suppression was accompanied by a reduction in transmitter release. The specificity of the nerve-muscle contact-induced Ca2+ rise was shown by its absence upon neuron-neuron contact. Naturally formed nerve-muscle contacts also showed an elevation in presynaptic Ca2+ level. Thus, this elevation appears to be a physiological step in the early stage of synaptogenesis and is likely mediated by muscle-derived molecules.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0896-6273
pubmed:author
pubmed:issnType
Print
pubmed:volume
10
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
827-37
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Elevation in presynaptic Ca2+ level accompanying initial nerve-muscle contact in tissue culture.
pubmed:affiliation
Department of Cell Biology and Anatomy and Curriculum in Neurobiology, University of North Carolina, Chapel Hill 27599.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.