Linked Life Data

8095173

Source:http://linkedlifedata.com/resource/pubmed/id/8095173

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1993-4-6
pubmed:abstractText
Cultured retina cells from chick embryos took up [3H]putrescine and approx 10.8% of the incorporated amine was converted into [3H]GABA. The putrescine-derived GABA accumulated in a pool that was released in the medium at a rate corresponding to 3.66% of the total [3H]GABA in the cell at incubation intervals of 12 min. Treatment of cultures with L-glutamate (500 microM) promoted a 5-7 fold increase in the rate of [3H]GABA efflux which was totally independent on the presence of calcium ions in the superfusing medium. (+)-5-Methyl-10,11-dihydro-5h-Dibenzo(A,D)cyclohepten-5,10- Iminihydrogenmaleate (MK 801) and 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX), 100 microM, inhibited the glutamate evoked release of GABA by 78 and 73% respectively. N-methyl-D-aspartate (NMDA, 100 microM), elicited the release of putrescine-derived GABA only when magnesium ions were removed from the superfusing medium with 2 mM EGTA. In the presence of 1 mM MgCl2, NMDA was totally ineffective in inducing the release. As for glutamate, AMPA (R,S)-alpha-Amino-3-hydroxy-5-methyllisoxazole-4-propionicacid+ ++ hydrobromide (100 microM) also induced the release of GABA synthesized from putrescine. Our data show that putrescine is an important source of GABA in the embryonic CNS and that GABA synthesized from putrescine can be released in the extracellular space when cells are stimulated by L-glutamate through the activation of excitatory amino acid (EAA) receptors.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0197-0186
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
249-53
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Excitatory amino acid receptors mediate the glutamate-induced release of GABA synthesized from putrescine in cultured cells of embryonic avian retina.
pubmed:affiliation
Instituto de Biofisica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't