Linked Life Data

8085156

Source:http://linkedlifedata.com/resource/pubmed/id/8085156

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5179
pubmed:dateCreated
1994-10-12
pubmed:abstractText
The opposing effects of SF2/ASF and heterogeneous nuclear ribonucleoprotein (hnRNP) A1 influence alternative splicing in vitro. SF2/ASF or hnRNP A1 complementary DNAs were transiently overexpressed in HeLa cells, and the effect on alternative splicing of several cotransfected reporter genes was measured. Increased expression of SF2/ASF activated proximal 5' splice sites, promoted inclusion of a neuron-specific exon, and prevented abnormal exon skipping. Increased expression of hnRNP A1 activated distal 5' splice sites. Therefore, variations in the intracellular levels of antagonistic splicing factors influence different modes of alternative splicing in vivo and may be a natural mechanism for tissue-specific or developmental regulation of gene expression.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0036-8075
pubmed:author
pubmed:issnType
Print
pubmed:day
16
pubmed:volume
265
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1706-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:8085156-Adenovirus E1A Proteins, pubmed-meshheading:8085156-Alternative Splicing, pubmed-meshheading:8085156-Base Sequence, pubmed-meshheading:8085156-DNA, Complementary, pubmed-meshheading:8085156-Exons, pubmed-meshheading:8085156-Gene Expression Regulation, pubmed-meshheading:8085156-Genes, Reporter, pubmed-meshheading:8085156-HeLa Cells, pubmed-meshheading:8085156-Heterogeneous-Nuclear Ribonucleoprotein Group A-B, pubmed-meshheading:8085156-Heterogeneous-Nuclear Ribonucleoproteins, pubmed-meshheading:8085156-Humans, pubmed-meshheading:8085156-Molecular Sequence Data, pubmed-meshheading:8085156-Nuclear Proteins, pubmed-meshheading:8085156-RNA-Binding Proteins, pubmed-meshheading:8085156-Ribonucleoproteins, pubmed-meshheading:8085156-Transfection, pubmed-meshheading:8085156-Tropomyosin
pubmed:year
1994
pubmed:articleTitle
Regulation of alternative splicing in vivo by overexpression of antagonistic splicing factors.
pubmed:affiliation
Cold Spring Harbor Laboratory, NY 11724.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't