Linked Life Data

8083517

Source:http://linkedlifedata.com/resource/pubmed/id/8083517

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1994-10-12
pubmed:abstractText
One of the major problems encountered during quantitative studies of phagocytosis is the discrimination without ambiguity between intracellular and extracellular particles. This difficulty is especially acute when zymosan particles are used because of their poor affinity for dyes. We show in this paper that zymosan particles may be stained by a mixture of a basic dye and tannic acid in water (or in an isotonic non saline solution). Crystal violet is one of the most suitable dyes and by combining this staining step with May-Grünwald Giemsa staining, it was possible to observe two populations of macrophage-associated particles. One comprises blue violet particles (BVP), and the second consists of particles with a purple-stained core (PPC). Treating macrophages in culture with various concentrations of cytochalasin B decreases the number of PPC and increases the number of BVP, in a dose dependant manner. Moreover, treatment with alpha-mannan or laminarin decreases the number of cell-associated particles, especially that of PPC. From these observations we concludes that BVP are extracellularly located and PPC are ingested.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0022-1759
pubmed:author
pubmed:issnType
Print
pubmed:day
14
pubmed:volume
174
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
155-65
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
A new method for studying the binding and ingestion of zymosan particles by macrophages.
pubmed:affiliation
Université Louis Pasteur, Département d'Immunologie, Immunopharmacologie et Pathologie, Illkirch, France.
pubmed:publicationType
Journal Article, In Vitro