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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1994-10-12
|
| pubmed:abstractText |
We have studied, at the mRNA level, the influence of various defined growth conditions on the expression of TGF-alpha, PDGF-BB, EGF-R, PDGF-R alpha, and PDGF-R beta in five different glioma cell lines (D-37MG, D-54MG, D-263MG, GaMG, and U-251MG). RNA isolated from logarithmically growing, confluent monolayer cells or multicellular spheroids was analyzed. Northern blot experiments show that with a few exceptions, specific mRNA steady-state levels were considerably higher in cells grown in a three-dimensional organization relative to cells in the logarithmic growth phase.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0014-4827
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
214
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
433-6
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:year |
1994
|
| pubmed:articleTitle |
Three-dimensional growth of glial cell lines affects growth factor and growth factor receptor mRNA levels.
|
| pubmed:affiliation |
Department of Biochemistry and Molecular Biology, University of Bergen, Norway.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|