Linked Life Data

8078518

Source:http://linkedlifedata.com/resource/pubmed/id/8078518

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-10-6
pubmed:abstractText
Although it seems clear that the trypanolytic factor in human serum capable of killing Trypanosoma brucei brucei is high density lipoprotein (HDL), it nevertheless remains controversial as to whether the trypanolytic properties of HDL are confined to a specific subclass or whether all particles have activity. In the present study, we have compared the lytic activities of serum fractions from six normal individuals prepared by gradient ultracentrifugation and also, to avoid ultracentrifugally-induced loss of HDL apolipoproteins, by gel filtration using fast protein liquid chromatography (FPLC). All sera displayed trypanolytic activity in fractions corresponding to the general density (rho = 1.06-1.20 g ml-1) and size (59-440 kDa) limits conventionally used to describe bulk human HDL, the particles between rho = 1.18-1.20 g ml-1 and between 214-440 kDa being particularly lytic. But some sera additionally contained fractions with powerful activity outside these density (rho > 1.24 g ml-1) and size (> 1000 kDa) ranges. Nevertheless, such fractions were considered to contain material with HDL characteristics; apolipoprotein A-I, the major protein of HDL, was always present and the lytic activity of the sera could be completely neutralized by absorption with HDL antiserum. We conclude that all of the trypanolytic activity in human sera is associated with HDL particles and that it is a property of several HDL subpopulations with very different density and size characteristics. Presumably the well-recognized wide variation in trypanocidal activity of normal human sera reflects differences in the quantities of these HDL subpopulations rather than in the total amount of a single, uniquely lytic particle.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0166-6851
pubmed:author
pubmed:issnType
Print
pubmed:volume
64
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
153-64
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Heterogeneity in the properties of the trypanolytic factor in normal human serum.
pubmed:affiliation
Swiss Tropical Institute, Basel.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't