|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1975-9-24
|
|
pubmed:abstractText |
The EGTA (ethanedioxybis(ethylamine)tetra-acetic acid)-Ruthenium Red-quench technique (Reed & Bygrave, 1974a) was used to measure initial rates of Ca-2+ transport in mitochondria from flight muscle of the blowfly Lucilia cuprina. Evidence is provided for the existence in these mitochondria of a Ca-2+-transport system that has many features in common with that known to exist in rat liver mitochondria. These include requirement for energy, saturation at high concentrations of Ca-2+, a sigmoidal relation between initial rates of Ca-2+ transport and Ca-2+ concentration, a high affinity for free Ca-2+ (Km approx. 5 muM) and high affinity for the Ca-2+-transport inhibitoy, Ruthenium Red (approx. 0.03 nmol of carrier-specific binding-sites/mg of protein; Ki approx. 1.6 x 10- minus 8 M). Controlled respiration can be stimulated by Ca-2+ after a short lag-period provided the incubation medium contains KCl and not sucrose. The ability of Lucilia mitochondria to transport Ca-2+ critically depends on the stage of mitochondrial development; Ca-2+ transport is minimal in mitochondria from pharate adults, is maximal between 0 and 2h post-emergence and thereafter rapidly declines to reach less than 20% of the maximum value by about 2-3 days post-emergence. Respiration in mitochondria from newly emerged flies does not respond to added Ca-2+; that from 3-5-day-old flies is stimulated approx. 50%. Whereas very low concentrations of Ca-2+ inhibit ADP-stimulated respiration and oxidative phosphorylation in mitochondria from newly emerged flies (Ki approx. 60 ng-ions of Ca-2+/mg of protein); much higher concentrations (approx. 200 ng-ion/mg of protein) are needed to inhibit these processes in those from older flies. The potential of this system for studying the function and development of metabolite transport systems in mitochondria is discussed.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/807204-13242552,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807204-14087334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807204-14304890,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807204-4108333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807204-4219276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807204-4250976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807204-4257941,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807204-4263188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807204-4274304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807204-4375957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807204-4415579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807204-4741658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807204-4768910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807204-4836276,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807204-4858265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807204-4964598,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807204-4980133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807204-5080728,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807204-5129264,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807204-5279045,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807204-5543696,
http://linkedlifedata.com/resource/pubmed/commentcorrection/807204-5767703
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0264-6021
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
146
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
601-8
|
|
pubmed:dateRevised |
2010-9-3
|
|
pubmed:meshHeading |
pubmed-meshheading:807204-Adenosine Diphosphate,
pubmed-meshheading:807204-Age Factors,
pubmed-meshheading:807204-Animals,
pubmed-meshheading:807204-Biological Transport, Active,
pubmed-meshheading:807204-Calcium,
pubmed-meshheading:807204-Diptera,
pubmed-meshheading:807204-Egtazic Acid,
pubmed-meshheading:807204-Kinetics,
pubmed-meshheading:807204-Mitochondria, Liver,
pubmed-meshheading:807204-Mitochondria, Muscle,
pubmed-meshheading:807204-Muscles,
pubmed-meshheading:807204-Oxidative Phosphorylation,
pubmed-meshheading:807204-Oxygen Consumption,
pubmed-meshheading:807204-Potassium Chloride,
pubmed-meshheading:807204-Rats,
pubmed-meshheading:807204-Ruthenium Red,
pubmed-meshheading:807204-Uncoupling Agents
|
|
pubmed:year |
1975
|
|
pubmed:articleTitle |
Evidence of a calcium-ion-transport system in mitochondria isolated from flight muscle of the developing sheep blowfly Lucilia cuprina.
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro
|
