Linked Life Data

8062107

Source:http://linkedlifedata.com/resource/pubmed/id/8062107

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-9-20
pubmed:abstractText
1. Embryos of the domestic fowl have been partially sterilised by injecting the drug busulphan into 24-h incubated eggs. 2. Some of these embryos were injected with primordial germ cells (PGCs) after 55 h of incubation to attempt to repopulate the gonads. 3. Primordial germ cells transfected with a defective retrovirus containing the reporter gene lac Z were shown to settle in these sterilised gonads. 4. Quantitative histology of 6-d embryos showed that busulphan produced 75% sterilisation but that PGCs could repopulate these gonads. 5. The technique of producing such germ line chimaeras is of value in studying cell kinetics, gonad differentiation and the production of transgenics.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0007-1668
pubmed:author
pubmed:issnType
Print
pubmed:volume
35
pubmed:geneSymbol
lac Z
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
241-7
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Manipulations of germ-cell populations in the gonad of the fowl.
pubmed:affiliation
Department of Pure and Applied Zoology, University of Reading, England.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't