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rdf:type |
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lifeskim:mentions |
umls-concept:C0012854,
umls-concept:C0026882,
umls-concept:C0040649,
umls-concept:C0085472,
umls-concept:C0442805,
umls-concept:C1510827,
umls-concept:C1514562,
umls-concept:C1551356,
umls-concept:C1553422,
umls-concept:C1704735,
umls-concept:C1705273,
umls-concept:C1705274,
umls-concept:C1705275,
umls-concept:C1706201,
umls-concept:C1709850,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221,
umls-concept:C2827501
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pubmed:issue |
1
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pubmed:dateCreated |
1994-9-13
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pubmed:abstractText |
We fused the wild-type Agrobacterium tumefaciens virG gene and the constitutive virGN54D allele to the malE gene of Escherichia coli, and studied the binding of MBP-VirG fusions to the autoregulated virG promoter. MBP-VirGN54D protein bound this promoter with 10-fold higher affinity than MBP-VirG, and bound to vir box I with eightfold higher affinity than to vir box III. Disruption of vir box III did not alter the affinity for vir box I, suggesting a lack of cooperativity between these sites. We provide evidence that protein bound at a single vir box may have a higher oligomeric state than non-bound protein, and that a DNA distortion adjacent to vir box I may occur during activation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0950-382X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:geneSymbol |
malE,
vir
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
23-30
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:8057837-Agrobacterium tumefaciens,
pubmed-meshheading:8057837-Bacterial Proteins,
pubmed-meshheading:8057837-Base Sequence,
pubmed-meshheading:8057837-DNA, Bacterial,
pubmed-meshheading:8057837-DNA-Binding Proteins,
pubmed-meshheading:8057837-Escherichia coli,
pubmed-meshheading:8057837-Gene Expression Regulation, Bacterial,
pubmed-meshheading:8057837-Genes, Bacterial,
pubmed-meshheading:8057837-Genes, Regulator,
pubmed-meshheading:8057837-Molecular Sequence Data,
pubmed-meshheading:8057837-Operator Regions, Genetic,
pubmed-meshheading:8057837-Phosphoproteins,
pubmed-meshheading:8057837-Phosphorylation,
pubmed-meshheading:8057837-Promoter Regions, Genetic,
pubmed-meshheading:8057837-Protein Binding,
pubmed-meshheading:8057837-Protein Processing, Post-Translational,
pubmed-meshheading:8057837-Recombinant Fusion Proteins,
pubmed-meshheading:8057837-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:8057837-Transcription Factors
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pubmed:year |
1994
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pubmed:articleTitle |
A mutation in the receiver domain of the Agrobacterium tumefaciens transcriptional regulator VirG increases its affinity for operator DNA.
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pubmed:affiliation |
Section of Microbiology, Cornell University, Ithaca, New York 14853.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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