Linked Life Data

8055649

Source:http://linkedlifedata.com/resource/pubmed/id/8055649

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1994-9-15
pubmed:abstractText
The degree of phosphorylation of c-Jun, Jun-B, Jun-D and Egr-1 transcription factors was examined during normal growth and during a prolonged period of defined transformation of NIH-3T3 cells which conditionally express v-sis [Mercola, D. et al. (1992) Oncogene, 7, 1793-1803]. During the asynchronous growth of normal cells phosphorylation of all factors was low and constant at all stages of growth from low density (c. 25 x 10(3) cells/cm2) through log-phase of growth to saturation density (c. 100 x 10(3) cells/cm2). Upon induction of v-sis, a marked and coordinate increase in phosphorylation occurred for c-Jun, Jun-B and Egr-1 to approximately 320%, 230% and 420% respectively above basal levels which was stable for the 2.5 day transformation period. The phosphorylation of Jun-D increased to over 600% and, after about 20 h, steadily declined to near basal levels at 54 h post-induction. Moreover, at any time phosphorylation and v-sis expression were fully reversible upon removal of the inducer. It appears that increased phosphorylation of the Jun family members and Egr-1 is not necessary for normal growth of NIH-3T3 but is dependent upon the expression of v-sis. Thus, normal and transformed cells may be distinguished. For c-Jun, the v-sis enhanced phosphorylation occurs at serines 63 and 73 and is required for transformation by several oncogenes [Smeal, T. et al. (1992) Mol. Cell. Biol., 12, 3507-3513]. The results described here show that the phosphorylation of additional factors is a stable and specific correlate of transformation which have have regulatory significance during transformation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0143-3334
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1667-74
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:8055649-3T3 Cells, pubmed-meshheading:8055649-Amino Acid Sequence, pubmed-meshheading:8055649-Animals, pubmed-meshheading:8055649-Cell Cycle, pubmed-meshheading:8055649-Cell Line, Transformed, pubmed-meshheading:8055649-Cell Transformation, Neoplastic, pubmed-meshheading:8055649-DNA-Binding Proteins, pubmed-meshheading:8055649-Early Growth Response Protein 1, pubmed-meshheading:8055649-Humans, pubmed-meshheading:8055649-Immediate-Early Proteins, pubmed-meshheading:8055649-Mice, pubmed-meshheading:8055649-Molecular Sequence Data, pubmed-meshheading:8055649-Oncogene Proteins v-sis, pubmed-meshheading:8055649-Oncogenes, pubmed-meshheading:8055649-Phosphorylation, pubmed-meshheading:8055649-Platelet-Derived Growth Factor, pubmed-meshheading:8055649-Proto-Oncogene Proteins c-jun, pubmed-meshheading:8055649-Retroviridae Proteins, Oncogenic, pubmed-meshheading:8055649-Transcription Factors
pubmed:year
1994
pubmed:articleTitle
Transformation-specific pattern of phosphorylation of c-Jun, Jun-B, Jun-D and Egr-1 in v-sis transformed cells.
pubmed:affiliation
San Diego Regional Cancer Center, CA 92161.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.