Linked Life Data

8054538

Source:http://linkedlifedata.com/resource/pubmed/id/8054538

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-9-9
pubmed:abstractText
Many T cell surface proteins are attached to the cell membrane by glycosylphosphatidylinositol (GPI) anchors, which may be involved in cell signaling, protein targeting or protein release. Proteins destined to be GPI-anchored have both N- and C-terminal signal peptides, which permit the nascent polypeptides to transverse the endoplasmic reticulum and to be transferred to preformed GPI anchors in a transamidase reaction. The biosynthetic pathway of the GPI anchor in mammalian cells has been elucidated using a panel of T cell mutants that cannot express GPI-anchored proteins on their cell surfaces. The first step in anchor biosynthesis is the transfer of N-acetylglucosamine (GlcNAc) to a phosphoinositol (PI) acceptor to form GlcNAc-PI, which is then deacetylated to form GlcN-PI. Subsequently, a fatty acid is added to the inositol ring and three mannose residues are transferred to the elongating GPI core. Finally, ethanolamine phosphates are transferred to the mannose residues. Three human cDNAs encoding for GPI synthases (Classes A, F and H) have been identified using expression cloning technique. Mutation in the X-linked class A gene was shown to be the cause of paroxysmal nocturnal hemoglobinuria, an acquired disease affecting hematopoietic stem cells in which the abnormal cells are deficient in GlcNAc-PI formation. The potential involvement of GPI-anchored proteins or GPIs in T cell activation is also discussed.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1044-5323
pubmed:author
pubmed:issnType
Print
pubmed:volume
6
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
73-80
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Biosynthesis and processing of the glycosylphosphatidylinositol anchor in mammalian cells.
pubmed:affiliation
Department of Medicine and Anesthesia, University of Texas, Houston Health Science Center, 77030.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Review, Research Support, Non-U.S. Gov't