Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1994-9-7
|
| pubmed:abstractText |
A cis-acting DNA regulatory element 3' to the A gamma-globin gene contains eight distinct regions of DNA-protein interaction distributed over 750 bp of DNA. The sequences of two foot-printed regions (sites I and IV) are A-T rich and generate a highly retarded complex on gel shift analysis with nuclear extract from human erythroleukemia (K562) cells. We have purified a 98-kD protein that reproduces this gel shift. Tryptic cleavage and peptide sequence analysis demonstrated that the 98-kD protein is identical to a recently cloned protein, special A-T-rich binding protein 1 (SATB1), that binds selectively to nuclear matrix/scaffold-associated regions of DNA (MARs/SARs). We have shown by functional analysis that the 3' A gamma regulatory element associates with the nuclear matrix. SATB1 mRNA was identified in K562 cells, and reverse transcriptase-polymerase chain reaction (RT-PCR) demonstrated its transcript in several other hematopoietic lines. Antisera to SATB1 caused ablation of the gel shift complex generated by both the crude nuclear extract and the purified 98-kD protein with the site I oligonucleotide. Furthermore, oligonucleotides that bind SATB1 inhibited formation of the site I gel shift complex when added as excess unlabeled competitor. An immunoblot analysis of the site I gel shift complex documented the presence of SATB1. Binding of SATB1 to two sites within the 3' A gamma regulatory element and its MAR/SAR activity suggests that this element may influence gene expression through interaction with the nuclear matrix.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Globins,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Attachment Region Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/SATB1 protein, human
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0006-4971
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
84
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1298-308
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:8049444-Amino Acid Sequence,
pubmed-meshheading:8049444-Base Sequence,
pubmed-meshheading:8049444-Binding Sites,
pubmed-meshheading:8049444-Cell Line,
pubmed-meshheading:8049444-DNA-Binding Proteins,
pubmed-meshheading:8049444-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:8049444-Globins,
pubmed-meshheading:8049444-Humans,
pubmed-meshheading:8049444-Leukemia, Erythroblastic, Acute,
pubmed-meshheading:8049444-Matrix Attachment Region Binding Proteins,
pubmed-meshheading:8049444-Molecular Sequence Data,
pubmed-meshheading:8049444-Mutagenesis,
pubmed-meshheading:8049444-Nuclear Matrix,
pubmed-meshheading:8049444-Oligodeoxyribonucleotides,
pubmed-meshheading:8049444-Peptide Fragments,
pubmed-meshheading:8049444-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:8049444-Tumor Cells, Cultured
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
The regulatory element 3' to the A gamma-globin gene binds to the nuclear matrix and interacts with special A-T-rich binding protein 1 (SATB1), an SAR/MAR-associating region DNA binding protein.
|
| pubmed:affiliation |
Clinical Hematology Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD.
|
| pubmed:publicationType |
Journal Article
|