|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
7
|
|
pubmed:dateCreated |
1975-7-2
|
|
pubmed:abstractText |
Microsomal 3-hydroxy-3-methylglutaryl coenzyme A reductase prepared from either rat liver or human fibroblasts was shown to be inactivated in vitro by a factor in extracts of cultured human fibroblasts in a reaction requiring ATP or ADP and Mg2+ or Mn2+. The inactivation factor was found in the soluble fraction of fibroblasts extracts and was shown to be heat-labile, nondialyzable, and precipitable with ammonium sulfate. The inactivation reaction was prevented by the addition of either an ATP-regenerating system or an ADP-regenerating system, suggesting that the presence of both adenine nucleotides may be required. A series of kinetic experiments suggested that the fibroblast inactivation factor catalyzes the conversion of the microsomal enzyme from an active to an inactive form. The finding that solubilized preparations of 3-hydroxy-3-methylglutaryl coenzyme A reductase from both rat liver and human fibroblasts were resistant to inactivation raises the possibility that some unidentified microsomal component also may participate in the inactivation reaction.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Apr
|
|
pubmed:issn |
0021-9258
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
10
|
|
pubmed:volume |
250
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
2502-9
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:804475-Adenosine Diphosphate,
pubmed-meshheading:804475-Adenosine Triphosphate,
pubmed-meshheading:804475-Alcohol Oxidoreductases,
pubmed-meshheading:804475-Animals,
pubmed-meshheading:804475-Cells, Cultured,
pubmed-meshheading:804475-Drug Stability,
pubmed-meshheading:804475-Edetic Acid,
pubmed-meshheading:804475-Enzyme Activation,
pubmed-meshheading:804475-Fibroblasts,
pubmed-meshheading:804475-Freezing,
pubmed-meshheading:804475-Glutarates,
pubmed-meshheading:804475-Hot Temperature,
pubmed-meshheading:804475-Humans,
pubmed-meshheading:804475-Kinetics,
pubmed-meshheading:804475-Magnesium,
pubmed-meshheading:804475-Male,
pubmed-meshheading:804475-Manganese,
pubmed-meshheading:804475-Microsomes,
pubmed-meshheading:804475-Microsomes, Liver,
pubmed-meshheading:804475-Proteins,
pubmed-meshheading:804475-Rats,
pubmed-meshheading:804475-Skin,
pubmed-meshheading:804475-Time Factors
|
|
pubmed:year |
1975
|
|
pubmed:articleTitle |
Inactivation of 3-hydroxy-3-methylglutaryl coenzyme A reductase in vitro. An adenine nucleotide-dependent reaction catalyzed by a factor in human fibroblasts.
|
|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
