803920

pubmed:Citation

1

The parameters of a persistent-state measles virus infection in BGM/MV cells were examined. The BGM/MV cell line was established by cocultivation of measles virus-infected primary C3H mouse brain cells with a stable line of African green monkey kidney cells (BGM). Initially, a morphologically mixed population of cells existed:BGM-like (epithelioid) and fibroblasts. Gradually the fibroblasts were replaced by BGM-like cells, resulting in a morphologically homogeneous population. Measles cytopathic effect was noted 2 days after initiation of this culture and persisted for approximately 290 days. The time of disappearance of viral cytopathic effect corresponded to the time at which morphological homogeneity was reached. Low titers of infectious measles virus were detected in the BGM/MV culture up to 20 days postseeding; thereafter none was observed. After 440 days in culture, 100% of BGM/MV cells demonstrated intractyoplasmic measles antigen by immunofluorescence. Nuclear fluorescence was never observed. Electron microscopy revealed the presence of measles virus mucleocapsid within the almost completely filling the cytoplasm of BGM/MV cells. The plasma membrane of these cells appeared normal; no maturing or budding particles were observed. Measles virus hemagglutinin was not detected in either clarified cell lysates or in supernatant culture fluids. Cell membrane alteration by measles virus was detected in less than 1% of these cells by hemadsorption and by membrane immunofluorescence. The hemadsorption activity of the cells could be enhanced (30 to 70%) by treatment with actinomycin D or enucleation with cytochalasin B; these treatments, however, were unsuccessful in inducing detectable levels of measles hemagglutinin. Treatment of BGM/MV cells with 5-bromo-2'-deoxyuridine (BUdR) at 5 to 50 mug/ml and cytosine arabinoside at 1 to 50 mug/ml failed to enhance hemadsorption activity. Doses of 5-bromo-2'-deoxyuridine ranging from 5 to 200 mug/ml and of actinomycin D ranging from 0.1 to 10 mug/ml were ineffective in inducing the synthesis of infectious virus. Various physical methods of induction of infectious virus was also unsuccessful.

eng

IM

MEDLINE

0019-9567

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NLM

152-8

pubmed-meshheading:803920-Animals, pubmed-meshheading:803920-Brain, pubmed-meshheading:803920-Bromodeoxyuridine, pubmed-meshheading:803920-Cell Line, pubmed-meshheading:803920-Cell Membrane, pubmed-meshheading:803920-Cell Nucleus, pubmed-meshheading:803920-Culture Techniques, pubmed-meshheading:803920-Cytarabine, pubmed-meshheading:803920-Cytochalasin B, pubmed-meshheading:803920-Dactinomycin, pubmed-meshheading:803920-Erythrocytes, pubmed-meshheading:803920-Fluorescent Antibody Technique, pubmed-meshheading:803920-Haplorhini, pubmed-meshheading:803920-Hemadsorption, pubmed-meshheading:803920-Hemagglutination Tests, pubmed-meshheading:803920-Kidney, pubmed-meshheading:803920-Measles virus, pubmed-meshheading:803920-Mice, pubmed-meshheading:803920-Mice, Inbred C3H, pubmed-meshheading:803920-Microscopy, Electron, pubmed-meshheading:803920-Radiation Effects, pubmed-meshheading:803920-Ultraviolet Rays, pubmed-meshheading:803920-Viral Plaque Assay

Characterization of an in vitro persistent-state measles virus infection: establishment and virological characterization of the BGM/MV cell line.