Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1994-8-15
pubmed:abstractText
The [Tyr5,12,Lys7]-polyphemusin II peptide (T22) has been shown to inhibit HIV-1 replication in lymphocytes. The mechanism of T22 inhibition of HIV-1 replication is not known but may involve T22 competition with HIV-1 for attachment sites on the plasma membrane of targeted cells. Here we find that three human immunocyte cell lines (H9, Jurkat, and U-937) attach to T22. The phorbol ester, 12-O-tetradecanoylphorbol 13-acetate (TPA), has been shown to activate intracellular protein kinase C and to stimulate lymphocyte attachment to various substrates through specific cell surface receptors. Here we find that TPA treatment enhances attachment of the immunocytes to T22 by three- to four-fold. These data demonstrate that T22 binds to immunocyte cell surfaces and support the hypothesis that T22 may inhibit HIV-1 replication by competing with the virus for a common cell surface receptor(s).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0006-291X
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
202
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
470-5
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed-meshheading:8037749-1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine, pubmed-meshheading:8037749-Amino Acid Sequence, pubmed-meshheading:8037749-Antimicrobial Cationic Peptides, pubmed-meshheading:8037749-Cell Adhesion, pubmed-meshheading:8037749-Cell Line, pubmed-meshheading:8037749-HIV-1, pubmed-meshheading:8037749-Humans, pubmed-meshheading:8037749-Isoquinolines, pubmed-meshheading:8037749-Kinetics, pubmed-meshheading:8037749-Lymphocytes, pubmed-meshheading:8037749-Molecular Sequence Data, pubmed-meshheading:8037749-Monocytes, pubmed-meshheading:8037749-Peptides, pubmed-meshheading:8037749-Phorbol Esters, pubmed-meshheading:8037749-Piperazines, pubmed-meshheading:8037749-Tetradecanoylphorbol Acetate, pubmed-meshheading:8037749-Tumor Cells, Cultured, pubmed-meshheading:8037749-Virus Replication
pubmed:year
1994
pubmed:articleTitle
Lymphocytes and promonocytes attach to the synthetic [Tyr5,12, Lys7]- polyphemusin II peptide.
pubmed:affiliation
Department of Biology, Hamilton College, Clinton, New York 13323.
pubmed:publicationType
Journal Article