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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1994-7-25
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pubmed:databankReference |
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pubmed:abstractText |
The conversion of 3-13C-labelled pyruvate in an acetoin-producing clone from a Lactococcus lactis subsp. lactis biovar diacetylactis strain DSM 20384 plasmid bank in Escherichia coli was studied by 13C nuclear magnetic resonance analysis. The results showed that alpha-acetolactate was the first metabolic product formed from pyruvate, whereas acetoin appeared at a much slower rate and reached only low concentrations. This alpha-acetolactate production shows that the cells express the gene for alpha-acetolactate synthase (als). Nucleotide sequence analysis identified an open reading frame encoding a protein of 554 amino acids. The deduced amino acid sequence exhibits extensive similarities to those of known alpha-acetolactate synthases from both prokaryotes and eukaryotes. The als gene is expressed on a monocistronic transcriptional unit, which is transcribed from a promoter located just upstream of the coding region.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8017926-1069307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8017926-1400210,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8017926-1444428,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8017926-1514784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8017926-1558766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8017926-1624471,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8017926-16347608,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8017926-16348592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8017926-16348602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8017926-2198252,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8017926-2450522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8017926-2497708,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8017926-2792374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8017926-2989783,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8017926-3162770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8017926-3278689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8017926-3550695,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8017926-5636815,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8017926-6308579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8017926-6403500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8017926-6546423,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8017926-7685336
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0099-2240
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
60
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1390-4
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pubmed:dateRevised |
2010-9-10
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pubmed:meshHeading |
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pubmed:year |
1994
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pubmed:articleTitle |
Identification and characterization of the alpha-acetolactate synthase gene from Lactococcus lactis subsp. lactis biovar diacetylactis.
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pubmed:affiliation |
Unilever Research Laboratorium, AT Vlaardingen, The Netherlands.
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pubmed:publicationType |
Journal Article
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