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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6484
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pubmed:dateCreated |
1994-7-25
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pubmed:abstractText |
Platelet-derived growth factor receptor (PDGF-R) phosphorylation at tyrosines 740/751 and insulin receptor phosphorylation of insulin receptor substrate-1 effects the recruitment and activation of phosphatidylinositol-3-OH kinase (PI(3)K). Changes in PI(3)K activity correlate with cell growth but its downstream signal transducers are unknown. Activation of the 70/85K S6 kinases (pp70S6k) by serine phosphorylation results in 40S ribosomal protein S6 phosphorylation and is important for G1 cell-cycle transition in a variety of cells. Although receptor tyrosine kinases activate the microtubule-associated protein kinase cascade through SH2-/SH3-adaptor proteins, Sos and c-Ras, it is unclear how tyrosine kinases are coupled to the pp70S6k phosphorylation cascade. Here we report that PI(3)K mediates PDGF or insulin receptor signalling to pp70S6k. PI(3)K-mediated activation of pp70S6k is independent of conventional protein kinase C isoforms. Additionally, rapamycin blocks pp70S6k activation by all mitogens, without inhibiting PI(3)K, and acts downstream in this signalling system.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Androstadienes,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphotransferases (Alcohol Group...,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet-Derived Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Polyenes,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Platelet-Derived Growth...,
http://linkedlifedata.com/resource/pubmed/chemical/Ribosomal Protein S6 Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Sirolimus,
http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases,
http://linkedlifedata.com/resource/pubmed/chemical/wortmannin
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0028-0836
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
7
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pubmed:volume |
370
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
71-5
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:8015612-3T3 Cells,
pubmed-meshheading:8015612-Androstadienes,
pubmed-meshheading:8015612-Animals,
pubmed-meshheading:8015612-Cell Line,
pubmed-meshheading:8015612-Enzyme Activation,
pubmed-meshheading:8015612-Insulin,
pubmed-meshheading:8015612-Mice,
pubmed-meshheading:8015612-Mutation,
pubmed-meshheading:8015612-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:8015612-Phosphotransferases (Alcohol Group Acceptor),
pubmed-meshheading:8015612-Platelet-Derived Growth Factor,
pubmed-meshheading:8015612-Polyenes,
pubmed-meshheading:8015612-Protein Kinase C,
pubmed-meshheading:8015612-Protein-Serine-Threonine Kinases,
pubmed-meshheading:8015612-Receptor, Insulin,
pubmed-meshheading:8015612-Receptors, Platelet-Derived Growth Factor,
pubmed-meshheading:8015612-Ribosomal Protein S6 Kinases,
pubmed-meshheading:8015612-Signal Transduction,
pubmed-meshheading:8015612-Sirolimus,
pubmed-meshheading:8015612-Type C Phospholipases
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pubmed:year |
1994
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pubmed:articleTitle |
PDGF- and insulin-dependent pp70S6k activation mediated by phosphatidylinositol-3-OH kinase.
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pubmed:affiliation |
Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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