|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0007961,
umls-concept:C0033684,
umls-concept:C0037633,
umls-concept:C0201699,
umls-concept:C0680730,
umls-concept:C1148554,
umls-concept:C1382100,
umls-concept:C1546426,
umls-concept:C1548280,
umls-concept:C1704419,
umls-concept:C1706211
|
|
pubmed:issue |
25
|
|
pubmed:dateCreated |
1995-1-11
|
|
pubmed:abstractText |
This paper describes two methods to estimate the effective charge of a protein in solution by capillary electrophoresis and demonstrates these methods by using representative proteins. In one method, a "charge ladder"--a series of derivatives of a protein differing by known increments of charge but differing only minimally in hydrodynamic drag--is generated by covalent modification of the epsilon-amino groups of lysines with 4-sulfophenyl isothiocyanate or acetic anhydride. In the second method, the equivalent of a charge ladder is produced by noncovalent association of a protein with differently charged ligands. Analysis of the electrophoretic mobilities of the protein and its derivatives as a function of added charge allows the effective charge to be estimated for the unmodified protein. This type of analysis permits estimation of the effective charge of a protein without knowing its composition, structure, or amino acid sequence.
|
|
pubmed:grant |
|
|
pubmed:commentsCorrections |
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4-isothiocyanatobenzenesulfonate,
http://linkedlifedata.com/resource/pubmed/chemical/Acetic Anhydrides,
http://linkedlifedata.com/resource/pubmed/chemical/Benzenesulfonates,
http://linkedlifedata.com/resource/pubmed/chemical/Carbonic Anhydrases,
http://linkedlifedata.com/resource/pubmed/chemical/Insulin,
http://linkedlifedata.com/resource/pubmed/chemical/Isothiocyanates,
http://linkedlifedata.com/resource/pubmed/chemical/Lactalbumin,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Lysine,
http://linkedlifedata.com/resource/pubmed/chemical/Myoglobin,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Solutions,
http://linkedlifedata.com/resource/pubmed/chemical/Trypsin,
http://linkedlifedata.com/resource/pubmed/chemical/acetic anhydride
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0027-8424
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
6
|
|
pubmed:volume |
91
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
12027-30
|
|
pubmed:dateRevised |
2011-11-17
|
|
pubmed:meshHeading |
pubmed-meshheading:7991578-Acetic Anhydrides,
pubmed-meshheading:7991578-Animals,
pubmed-meshheading:7991578-Benzenesulfonates,
pubmed-meshheading:7991578-Capillary Action,
pubmed-meshheading:7991578-Carbonic Anhydrases,
pubmed-meshheading:7991578-Cattle,
pubmed-meshheading:7991578-Electrophoresis,
pubmed-meshheading:7991578-Horses,
pubmed-meshheading:7991578-Humans,
pubmed-meshheading:7991578-Insulin,
pubmed-meshheading:7991578-Isothiocyanates,
pubmed-meshheading:7991578-Lactalbumin,
pubmed-meshheading:7991578-Ligands,
pubmed-meshheading:7991578-Lysine,
pubmed-meshheading:7991578-Myoglobin,
pubmed-meshheading:7991578-Proteins,
pubmed-meshheading:7991578-Solutions,
pubmed-meshheading:7991578-Swine,
pubmed-meshheading:7991578-Trypsin
|
|
pubmed:year |
1994
|
|
pubmed:articleTitle |
Determination of the effective charge of a protein in solution by capillary electrophoresis.
|
|
pubmed:affiliation |
Department of Chemistry, Harvard University, Cambridge, MA 02138.
|
|
pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|
