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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
10
|
pubmed:dateCreated |
1995-1-4
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pubmed:abstractText |
The polymerase chain reaction-single stranded conformation Polymorphism (PCR-SSCP) procedure was applied to examine whether the mutation in the liver/islet glucose transporter (GLUT2) gene could be associated with non-insulin-dependent diabetes mellitus (NIDDM) in Japanese. Samples were processed through 30-40 cycles of 1 min denaturation at 94 degrees C, 1 min annealing at 55-68 degrees C for 1-2 min, extention at 72 degrees C for 1 min, and denaturation at 94 degrees C for 1 min. We identified a silent mutation in codon 479 for PheTTT/TTC.
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pubmed:language |
jpn
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Oct
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pubmed:issn |
0047-1852
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:volume |
52
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pubmed:geneSymbol |
GLUT2
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
2568-71
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pubmed:dateRevised |
2011-7-27
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pubmed:meshHeading |
pubmed-meshheading:7983779-Base Sequence,
pubmed-meshheading:7983779-Diabetes Mellitus, Type 2,
pubmed-meshheading:7983779-Humans,
pubmed-meshheading:7983779-Molecular Sequence Data,
pubmed-meshheading:7983779-Monosaccharide Transport Proteins,
pubmed-meshheading:7983779-Mutation,
pubmed-meshheading:7983779-Polymerase Chain Reaction,
pubmed-meshheading:7983779-Polymorphism, Genetic
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pubmed:year |
1994
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pubmed:articleTitle |
[The detection of GLUT2 gene mutation by polymerase-chain reaction single stranded conformation polymorphism (PCR-SSCP) method].
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pubmed:affiliation |
Department of Metabolism and Clinical Nutrition, Kyoto University Faculty of Medicine.
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pubmed:publicationType |
Journal Article,
English Abstract,
Review
|