| pubmed-article:7980874 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7980874 | lifeskim:mentions | umls-concept:C0935640 | lld:lifeskim |
| pubmed-article:7980874 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:7980874 | lifeskim:mentions | umls-concept:C0079784 | lld:lifeskim |
| pubmed-article:7980874 | lifeskim:mentions | umls-concept:C0205177 | lld:lifeskim |
| pubmed-article:7980874 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:7980874 | lifeskim:mentions | umls-concept:C0376315 | lld:lifeskim |
| pubmed-article:7980874 | lifeskim:mentions | umls-concept:C0037119 | lld:lifeskim |
| pubmed-article:7980874 | lifeskim:mentions | umls-concept:C0205460 | lld:lifeskim |
| pubmed-article:7980874 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:7980874 | lifeskim:mentions | umls-concept:C0323309 | lld:lifeskim |
| pubmed-article:7980874 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:7980874 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:7980874 | pubmed:dateCreated | 1995-1-5 | lld:pubmed |
| pubmed-article:7980874 | pubmed:abstractText | The human macrophage colony stimulating factor (hM-CSF) in its monomeric form has been over-produced in BmN cells and in silkworm larvae infected with the recombinant baculovirus Bm284M-CSF. The recombinant monomeric M-CSF (rhM-CSF) exhibited the activity of 8-14 x 10(4) units/ml of cell culture medium. When the insect larvae were infected with the recombinant virus, the maximum rhM-CSF was expressed 4-5 days post infection with an activity of 3 x 10(6) units/ml hemolymph. The monomeric rhM-CSF was purified to homogeneity through three steps of purification. A pilot purification yielded 1 mg of homogeneous monomeric rhM-CSF from 10 larvae. The purified rhM-CSF monomers gradually dimerized in vitro. In contrast, the crude or the semi-purified monomers did not dimerize in vitro, indicating that the presence of an unknown moiety in the rhM-CSF preparations obtained from hemolymph interfered with dimerization. | lld:pubmed |
| pubmed-article:7980874 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7980874 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7980874 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7980874 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7980874 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7980874 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7980874 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7980874 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7980874 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7980874 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:7980874 | pubmed:issn | 0177-3593 | lld:pubmed |
| pubmed-article:7980874 | pubmed:author | pubmed-author:CoxG HGH | lld:pubmed |
| pubmed-article:7980874 | pubmed:author | pubmed-author:ZhuDD | lld:pubmed |
| pubmed-article:7980874 | pubmed:author | pubmed-author:QinJJ | lld:pubmed |
| pubmed-article:7980874 | pubmed:author | pubmed-author:DingYY | lld:pubmed |
| pubmed-article:7980874 | pubmed:author | pubmed-author:HoQ YQY | lld:pubmed |
| pubmed-article:7980874 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7980874 | pubmed:volume | 375 | lld:pubmed |
| pubmed-article:7980874 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7980874 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7980874 | pubmed:pagination | 413-8 | lld:pubmed |
| pubmed-article:7980874 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
| pubmed-article:7980874 | pubmed:meshHeading | pubmed-meshheading:7980874-... | lld:pubmed |
| pubmed-article:7980874 | pubmed:meshHeading | pubmed-meshheading:7980874-... | lld:pubmed |
| pubmed-article:7980874 | pubmed:meshHeading | pubmed-meshheading:7980874-... | lld:pubmed |
| pubmed-article:7980874 | pubmed:meshHeading | pubmed-meshheading:7980874-... | lld:pubmed |
| pubmed-article:7980874 | pubmed:meshHeading | pubmed-meshheading:7980874-... | lld:pubmed |
| pubmed-article:7980874 | pubmed:meshHeading | pubmed-meshheading:7980874-... | lld:pubmed |
| pubmed-article:7980874 | pubmed:meshHeading | pubmed-meshheading:7980874-... | lld:pubmed |
| pubmed-article:7980874 | pubmed:meshHeading | pubmed-meshheading:7980874-... | lld:pubmed |
| pubmed-article:7980874 | pubmed:meshHeading | pubmed-meshheading:7980874-... | lld:pubmed |
| pubmed-article:7980874 | pubmed:meshHeading | pubmed-meshheading:7980874-... | lld:pubmed |
| pubmed-article:7980874 | pubmed:meshHeading | pubmed-meshheading:7980874-... | lld:pubmed |
| pubmed-article:7980874 | pubmed:meshHeading | pubmed-meshheading:7980874-... | lld:pubmed |
| pubmed-article:7980874 | pubmed:meshHeading | pubmed-meshheading:7980874-... | lld:pubmed |
| pubmed-article:7980874 | pubmed:meshHeading | pubmed-meshheading:7980874-... | lld:pubmed |
| pubmed-article:7980874 | pubmed:meshHeading | pubmed-meshheading:7980874-... | lld:pubmed |
| pubmed-article:7980874 | pubmed:meshHeading | pubmed-meshheading:7980874-... | lld:pubmed |
| pubmed-article:7980874 | pubmed:meshHeading | pubmed-meshheading:7980874-... | lld:pubmed |
| pubmed-article:7980874 | pubmed:meshHeading | pubmed-meshheading:7980874-... | lld:pubmed |
| pubmed-article:7980874 | pubmed:meshHeading | pubmed-meshheading:7980874-... | lld:pubmed |
| pubmed-article:7980874 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7980874 | pubmed:articleTitle | Expression of biologically active monomeric form of human M-CSF in baculovirus infected silkworm, Bombyx mori. | lld:pubmed |
| pubmed-article:7980874 | pubmed:affiliation | Department of Biochemistry, Nanjing University, P.R. China. | lld:pubmed |
| pubmed-article:7980874 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7980874 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7980874 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7980874 | lld:pubmed |
