Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1994-11-30
pubmed:abstractText
In the human insulin gene, three regulatory sequences upstream of the transcription start site at -77 (the CT1 box), -210 (the CT2 box), and -315 (the CT3 box) bind a beta-cell-specific transcription factor, IUF1. Recent studies have mapped a glucose response element to a CT-like sequence in the rat insulin I gene. The present study was therefore undertaken to ascertain the role of IUF1 in glucose-stimulated insulin gene transcription. IUF1-binding activity was measured by electrophoretic mobility shift assay using the CT2 box as probe. When freshly isolated rat islets of Langerhans were incubated in medium containing low concentrations (3 mM) of glucose IUF1 activity fell to undetectable levels within 6 h. In high (20 mM) glucose IUF1 activity remained constant over a 24 h period. The loss of IUF1 activity was reversible. Thus when islets were incubated for 4 h in low glucose and transferred to high glucose, IUF1 levels recovered within 15 min. This effect was dependent on glucose metabolism as it was inhibited by mannoheptulose. Incubation of islets for 4 h in low concentrations of glucose supplemented with phosphatase inhibitors prevented the fall in IUF1 activity. No recovery in IUF1 activity was observed when islets were treated for 4 h with low glucose and then for a further 1 h with low glucose and dibutyryl cyclic AMP, or forskolin, or the phorbol ester phorbol 12-myristate 13-acetate. These results demonstrate that the IUF1-binding activity in islets of Langerhans is modulated by glucose in a phosphorylation-dependent manner, and that protein kinase A or protein kinase C are not involved. Finally, IUF1 was shown to be immunologically related to a recently cloned factor, IPF1, that binds to a CT-like sequence in the rat insulin I gene promoter.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-1315961, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-1322137, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-1406648, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-1473318, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-1549125, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-1639803, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-1691825, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-1710033, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-1915886, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-1944296, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-1974136, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-1979979, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-2153135, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-2181401, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-2186040, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-2196879, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-2690822, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-2771659, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-3321054, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-4879533, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-6245006, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-6358900, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-7505393, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-7901001, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-8216223, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-8226928, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-8246989, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-8299887, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-8326016, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-8354388, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-8449283, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-8479910, http://linkedlifedata.com/resource/pubmed/commentcorrection/7980425-8483904
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
303 ( Pt 2)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
625-31
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:7980425-Animals, pubmed-meshheading:7980425-Base Sequence, pubmed-meshheading:7980425-Binding, Competitive, pubmed-meshheading:7980425-Blotting, Western, pubmed-meshheading:7980425-Bucladesine, pubmed-meshheading:7980425-Dose-Response Relationship, Drug, pubmed-meshheading:7980425-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:7980425-Enhancer Elements, Genetic, pubmed-meshheading:7980425-Forskolin, pubmed-meshheading:7980425-Glucose, pubmed-meshheading:7980425-Humans, pubmed-meshheading:7980425-Islets of Langerhans, pubmed-meshheading:7980425-Male, pubmed-meshheading:7980425-Mannoheptulose, pubmed-meshheading:7980425-Molecular Sequence Data, pubmed-meshheading:7980425-Oligonucleotide Probes, pubmed-meshheading:7980425-Phosphoric Monoester Hydrolases, pubmed-meshheading:7980425-Phosphorylation, pubmed-meshheading:7980425-Rats, pubmed-meshheading:7980425-Rats, Wistar, pubmed-meshheading:7980425-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:7980425-Transcription Factors
pubmed:year
1994
pubmed:articleTitle
Glucose modulates the binding activity of the beta-cell transcription factor IUF1 in a phosphorylation-dependent manner.
pubmed:affiliation
Department of Medicine, University of Birmingham, Queen Elizabeth Hospital, U.K.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't