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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-4
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pubmed:dateCreated |
1994-12-7
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pubmed:databankReference |
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pubmed:abstractText |
A 90 bp fragment prepared from the promoter region of the rat preprodynorphin gene formed a complex with rat brain nuclear extracts as assessed by gel mobility shift assays. An 8 base pair sequence, CACTCTCC, termed upstream regulatory element (URE), was identified within this fragment as a binding site by DNase 1 footprint analysis and gel mobility shift assays with synthetic oligonucleotides. The URE is a consensus sequence for a transcription initiator (Inr) element although in the preprodynorphin promoter it is located upstream at -208 and overlaps a region conserved between rat and human promoters. A unique 310 amino acid protein (UreB1) that specifically bound the URE was cloned from a rat brain cDNA library using the URE-containing oligonucleotide. Recombinantly expressed, affinity purified UreB1 protein retains specific binding to the URE oligonucleotide. UreB1 contains a tyrosine kinase phosphorylation consensus and binding is enhanced following phosphorylation with the p43v-abl tyrosine kinase. The UreB1 tyrosine phosphoprotein increases transcription in vitro, consistent with a positive transcriptional regulatory function. UreB1 transcripts are well expressed in subsets of neurons in multiple brain areas suggesting that, in addition to regulation of the preprodynorphin gene, it may have a more generalized role in gene transcription.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0169-328X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
77-88
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7968380-Amino Acid Sequence,
pubmed-meshheading:7968380-Animals,
pubmed-meshheading:7968380-Base Sequence,
pubmed-meshheading:7968380-Binding Sites,
pubmed-meshheading:7968380-Brain,
pubmed-meshheading:7968380-Cell Nucleus,
pubmed-meshheading:7968380-Cloning, Molecular,
pubmed-meshheading:7968380-DNA-Binding Proteins,
pubmed-meshheading:7968380-Dynorphins,
pubmed-meshheading:7968380-Escherichia coli,
pubmed-meshheading:7968380-Gene Expression,
pubmed-meshheading:7968380-Molecular Sequence Data,
pubmed-meshheading:7968380-Neurons,
pubmed-meshheading:7968380-Peripheral Nerves,
pubmed-meshheading:7968380-Phosphoproteins,
pubmed-meshheading:7968380-Phosphorylation,
pubmed-meshheading:7968380-Promoter Regions, Genetic,
pubmed-meshheading:7968380-Prosencephalon,
pubmed-meshheading:7968380-Protein Precursors,
pubmed-meshheading:7968380-Protein-Tyrosine Kinases,
pubmed-meshheading:7968380-Rats,
pubmed-meshheading:7968380-Recombinant Proteins,
pubmed-meshheading:7968380-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:7968380-Restriction Mapping,
pubmed-meshheading:7968380-Transcription, Genetic
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pubmed:year |
1994
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pubmed:articleTitle |
Cloning of a DNA binding protein that is a tyrosine kinase substrate and recognizes an upstream initiator-like sequence in the promoter of the preprodynorphin gene.
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pubmed:affiliation |
Neurobiology and Anesthesiology Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892.
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pubmed:publicationType |
Journal Article
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