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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
12
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pubmed:dateCreated |
1994-12-12
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pubmed:databankReference |
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pubmed:abstractText |
Hepatitis C virus (HCV) serine proteinase (Cpro-2) is responsible for the processing of HCV nonstructural (NS) protein processing. To clarify the mechanism of Cpro-2-dependent processing, pulse-chase and mutation analyses were performed by using a transient protein production system in cultured cells. Pulse-chase study revealed the sequential production of HCV-NS proteins. Production of p70(NS3) and p66(NS5B) were rapid. An 89-kDa processing intermediate protein (p89) was observed during the early part of the chase. p89 seemed to be cleaved first into a 31-kDa protein (p31) and a p58/56(NS5A). p31 was further processed into p4(NS4A) and p27(NS4B). Mutation analysis of cleavage sites of NS4A/4B, NS4B/5A, and NS5A/5B revealed that cleavage at each site is essentially independent from cleavage occurring at the other site.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7966638-1314449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7966638-1314459,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7966638-1318627,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7966638-1648221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7966638-1658196,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7966638-1847440,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7966638-1848704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7966638-2173727,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7966638-2449095,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7966638-2496467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7966638-3185726,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7966638-7504283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7966638-7685406,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7966638-8056334,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7966638-8056335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7966638-8139048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7966638-8291245,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7966638-8386278,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7966638-8387277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7966638-8389908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7966638-8392606
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0022-538X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
68
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8418-22
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7966638-Animals,
pubmed-meshheading:7966638-Cell Line,
pubmed-meshheading:7966638-Cercopithecus aethiops,
pubmed-meshheading:7966638-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:7966638-Hepacivirus,
pubmed-meshheading:7966638-Kidney,
pubmed-meshheading:7966638-Kinetics,
pubmed-meshheading:7966638-Methionine,
pubmed-meshheading:7966638-Molecular Sequence Data,
pubmed-meshheading:7966638-Mutagenesis, Site-Directed,
pubmed-meshheading:7966638-Open Reading Frames,
pubmed-meshheading:7966638-Protein Processing, Post-Translational,
pubmed-meshheading:7966638-Serine Endopeptidases,
pubmed-meshheading:7966638-Time Factors,
pubmed-meshheading:7966638-Transfection,
pubmed-meshheading:7966638-Viral Nonstructural Proteins
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pubmed:year |
1994
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pubmed:articleTitle |
Hepatitis C virus polyprotein processing: kinetics and mutagenic analysis of serine proteinase-dependent cleavage.
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pubmed:affiliation |
Virology Division, National Cancer Center Research Institute, Tokyo, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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