Linked Life Data

7963696

Source:http://linkedlifedata.com/resource/pubmed/id/7963696

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1994-12-1
pubmed:databankReference
http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U12492, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U12493, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U12494, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U12495, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U12496, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U12497, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U12498, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U12499, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U12500, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U12501, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U12502, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U12503, http://linkedlifedata.com/resource/pubmed/xref/GENBANK/U12504
pubmed:abstractText
The identification and taxonomy of papillomaviruses has become increasingly complex, as approximately 70 human papillomavirus (HPV) types have been described and novel HPV genomes continue to be identified. Methods and corresponding DNA sequence data bases were designed for the reliable identification of mucosal HPV genomes from clinical specimens. HPVs are identified by the amplification of a fragment of the L1 region by consensus primer polymerase chain reaction (PCR) and subsequent hybridization or restriction fragment length polymorphism analysis. L1 PCR fragments may be further characterized by nucleotide sequencing. Conservation of 30 (of 151) predicted amino acids identifies HPV genomic fragments, and nucleotide sequence alignments allow calculation of their phylogenetic relatedness. Sequence differences > 10% from any known HPV type suggest a novel HPV type. Phylogenetic relationships with known HPV types may permit predictions of biology. With these criteria, 10 PCR fragments were identified that would qualify as new genital HPV types after complete genomic isolation.
pubmed:grant
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-1899
pubmed:author
pubmed:issnType
Print
pubmed:volume
170
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1077-85
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
Identification and assessment of known and novel human papillomaviruses by polymerase chain reaction amplification, restriction fragment length polymorphisms, nucleotide sequence, and phylogenetic algorithms.
pubmed:affiliation
Laboratory for Papillomavirus Biology, National University of Singapore.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't