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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1994-12-22
|
| pubmed:databankReference | |
| pubmed:abstractText |
The hepatocyte nuclear factor-3 (HNF-3)/forkhead (fkh) proteins consist of an extensive family of tissue-specific and developmental gene regulators which share homology within the winged helix DNA binding motif. We report on the isolation of a new family member, HNF-3/forkhead homolog 8 (HFH-8), from lung cDNA libraries and the derivation of the complete amino acid sequences for the HFH-8 protein as well as previously identified HFH-1 and HFH-4 proteins. The HFH proteins contain several sequence motifs found in activation domains of other transcription factors and HNF-3/fkh family members. In situ hybridization with the HNF-3, HFH-4, and HFH-8 probes in adult lung demonstrate that the HNF-3/fkh cellular expression patterns are regionally specified. Whereas HNF-3 alpha and HNF-3 beta are normally coexpressed in the hepatocyte, their expression patterns in the lung are different. The HNF-3 alpha and HFH-4 genes are coexpressed in the bronchiolar epithelium (clara cells), whereas the HNF-3 beta probe exhibits prominent hybridization with the smooth muscle surrounding arterioles and bronchioles. In contrast, HFH-8 probes labeled the type II pneumocyte cells lining the respiratory surfaces of terminal bronchioles and alveolar sac. We have identified an HNF-3 consensus DNA binding sequence in the proximal surfactant protein B (SPB) promoter region (SPB-f2, -78 to -88). SPB gene transcription is restricted to bronchiolar and alveolar epithelium which colocalizes with the expression pattern of the HNF-3 alpha and HFH-8 genes, respectively. We show that the SPB-f2 sequence is recognized by both HNF-3 alpha and HFH-8 proteins and that these cDNA expression vectors activate the SPB promoter in cotransfection assays through the HNF-3 consensus sequence. Our results suggest that SPB promoter activity is regulated by HNF-3 alpha and HFH-8 proteins in a cell type-specific manner.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Forkhead Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteolipids,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactants,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0012-1606
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
166
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
195-209
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:7958446-Aging,
pubmed-meshheading:7958446-Amino Acid Sequence,
pubmed-meshheading:7958446-Animals,
pubmed-meshheading:7958446-Base Sequence,
pubmed-meshheading:7958446-Bronchi,
pubmed-meshheading:7958446-Embryonic and Fetal Development,
pubmed-meshheading:7958446-Epithelium,
pubmed-meshheading:7958446-Forkhead Transcription Factors,
pubmed-meshheading:7958446-Gene Expression,
pubmed-meshheading:7958446-Gene Expression Regulation,
pubmed-meshheading:7958446-Gene Library,
pubmed-meshheading:7958446-In Situ Hybridization,
pubmed-meshheading:7958446-Liver,
pubmed-meshheading:7958446-Lung,
pubmed-meshheading:7958446-Male,
pubmed-meshheading:7958446-Mice,
pubmed-meshheading:7958446-Mice, Inbred BALB C,
pubmed-meshheading:7958446-Molecular Sequence Data,
pubmed-meshheading:7958446-Nuclear Proteins,
pubmed-meshheading:7958446-Organ Specificity,
pubmed-meshheading:7958446-Promoter Regions, Genetic,
pubmed-meshheading:7958446-Proteolipids,
pubmed-meshheading:7958446-Pulmonary Alveoli,
pubmed-meshheading:7958446-Pulmonary Surfactants,
pubmed-meshheading:7958446-Rats,
pubmed-meshheading:7958446-Rats, Sprague-Dawley,
pubmed-meshheading:7958446-Recombinant Fusion Proteins,
pubmed-meshheading:7958446-Sequence Homology, Amino Acid,
pubmed-meshheading:7958446-Transcription Factors
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Members of the HNF-3/forkhead family of transcription factors exhibit distinct cellular expression patterns in lung and regulate the surfactant protein B promoter.
|
| pubmed:affiliation |
Department of Biochemistry (M/C 536), College of Medicine, University of Illinois at Chicago 60612-7334.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|