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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1977-3-31
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pubmed:abstractText |
A method enabling direct enumeration of mouse T and B cells from cytocentrifuged cell smears is described. The acid alpha-naphthyl acetate esterase (ANAE) staining was used as marker for T cells. The distribution of the ANAE marker-carrying lymphocytes closely followed the percentual distribution of T cells in spleen and lymph node. Electrophoresis analysis demonstrated that while more than 95% of resting spleen and lymph node T cells carried the ANAE marker, only less than 5% of the B cells were ANAE positive. The B cells (surface-bound Ig-carrying small lymphocytes) were identified by anti-Ig serum followed by rosetting of the B cells with Staphylococcus aureus strain Cowan 1 (StaCw). The ANAE marker-carrying cells did not overlap with the StaCw rosette-forming lymphocytes. Thus we conclude that the combination of StaCw rosetting and ANAE staining enables accurate identification of resting T and B cells from a single microscope slide. Ninety to 100% of Con A-activated blasts expressed the ANAE marker but only 60-85% of PHA and MLC-activated blasts were positive. Twenty to 33% of the blast cells stimulated by LPS expressed the ANAE marker. Thus the ANAE marker is not a reliable criterion for T cells in actibated state.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/795580-1086232,
http://linkedlifedata.com/resource/pubmed/commentcorrection/795580-4106485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/795580-4108877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/795580-4136792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/795580-4143965,
http://linkedlifedata.com/resource/pubmed/commentcorrection/795580-4268630,
http://linkedlifedata.com/resource/pubmed/commentcorrection/795580-4272900,
http://linkedlifedata.com/resource/pubmed/commentcorrection/795580-4501203,
http://linkedlifedata.com/resource/pubmed/commentcorrection/795580-4544080,
http://linkedlifedata.com/resource/pubmed/commentcorrection/795580-4554455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/795580-4559167,
http://linkedlifedata.com/resource/pubmed/commentcorrection/795580-4579962
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
26
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
632-40
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:795580-Animals,
pubmed-meshheading:795580-B-Lymphocytes,
pubmed-meshheading:795580-Carboxylic Ester Hydrolases,
pubmed-meshheading:795580-Cell Separation,
pubmed-meshheading:795580-Centrifugation,
pubmed-meshheading:795580-Cytological Techniques,
pubmed-meshheading:795580-Leukocyte Count,
pubmed-meshheading:795580-Lymphocyte Activation,
pubmed-meshheading:795580-Mice,
pubmed-meshheading:795580-Receptors, Antigen, B-Cell,
pubmed-meshheading:795580-T-Lymphocytes
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pubmed:year |
1976
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pubmed:articleTitle |
Identification of mouse T and B lymphocytes from cytocentrifuged cell smears.
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pubmed:publicationType |
Journal Article
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