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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1994-12-13
|
| pubmed:abstractText |
The effects of estrogen (17-beta estradiol) and antiestrogens (tamoxifen, clomiphene and nafoxidine) on the growth of SK-N-MC human neuroblastoma cells were investigated. At low concentrations these agents enhanced, but at high concentrations they inhibited, the growth of the tumor cells in a dose-dependent manner. The growth inhibition was found to be due to decreased cell viability. When serum-free media were used, the dose-response curves were left-shifted, indicating that these agents can directly act on the tumor cells and that serum factors can inhibit their growth-modulatory actions. Growth enhancement and decreased cell viability induced by these agents were significantly reversed by the treatments with either Ca(2+)-free media, intracellular Ca2+ release blockers (dantrolene or ruthenium red) or BAPTA/AM, an intracellular Ca2+ chelator, implying that both intracellular Ca2+ release and extracellular Ca2+ entry may play a role in their growth regulation. These results suggest that estrogen and antiestrogens have concentration-dependent dual effects on the growth of the tumor cells and that the mechanism of their actions may be through the interaction with intracellular Ca2+ signalling mechanisms.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0304-3835
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
28
|
| pubmed:volume |
86
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
119-25
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:7954348-Calcium,
pubmed-meshheading:7954348-Cell Division,
pubmed-meshheading:7954348-Culture Media, Serum-Free,
pubmed-meshheading:7954348-Drug Interactions,
pubmed-meshheading:7954348-Estradiol,
pubmed-meshheading:7954348-Estrogen Antagonists,
pubmed-meshheading:7954348-Humans,
pubmed-meshheading:7954348-Intracellular Fluid,
pubmed-meshheading:7954348-Kinetics,
pubmed-meshheading:7954348-Models, Biological,
pubmed-meshheading:7954348-Neuroblastoma,
pubmed-meshheading:7954348-Signal Transduction,
pubmed-meshheading:7954348-Tumor Cells, Cultured
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Dual effects of estrogen and antiestrogens on the growth of SK-N-MC human neuroblastoma cells.
|
| pubmed:affiliation |
Department of Neurological Surgery, Loyola University Medical Center, Maywood, IL 60153.
|
| pubmed:publicationType |
Journal Article
|