|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:dateCreated |
1994-11-9
|
|
pubmed:abstractText |
It has previously been reported that two kinds of human gastric adenocarcinoma cell lines (STKM-1 and MKN28) secrete a trypsin-like enzyme. In this study, four molecular forms of the enzyme (26, 25, 24 and 23 kDa on non-reducing SDS/PAGE) were purified from the serum-free conditioned medium of STKM-1 cells. Analysis of N-terminal amino acid sequences showed that the 26 kDa protein was a two-chain form of trypsinogen 1 which had been produced by proteolytic cleavage of the Arg107-Val108 bond of trypsinogen 1, and the 24 kDa protein was the one-chain form of trypsinogen 1. The 25 and 23 kDa proteins were the activated forms of the two-chain and one-chain trypsinogen 1 respectively. Isoelectric focusing gave pI values of 6.3 and 6.6 for the 26 kDa two-chain form and the 24 kDa one-chain form of trypsinogen 1 respectively. Comparison of the proteolytic activities indicated that the one-chain trypsin 1 had amidolytic activity about four times higher than that of the two-chain enzyme.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7945238-1079768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7945238-1318881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7945238-1387587,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7945238-1537555,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7945238-1662201,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7945238-1850705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7945238-1995487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7945238-2105839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7945238-2132731,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7945238-2180568,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7945238-2253219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7945238-3011602,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7945238-6069108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7945238-6349614,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7945238-656395,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7945238-8314798,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7945238-8340251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7945238-8479521,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7945238-8481902
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Oct
|
|
pubmed:issn |
0264-6021
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
303 ( Pt 1)
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
187-90
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:7945238-Adenocarcinoma,
pubmed-meshheading:7945238-Amino Acid Sequence,
pubmed-meshheading:7945238-Cell Line,
pubmed-meshheading:7945238-Chromatography, High Pressure Liquid,
pubmed-meshheading:7945238-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:7945238-Humans,
pubmed-meshheading:7945238-Kinetics,
pubmed-meshheading:7945238-Macromolecular Substances,
pubmed-meshheading:7945238-Molecular Sequence Data,
pubmed-meshheading:7945238-Peptide Fragments,
pubmed-meshheading:7945238-Protein Conformation,
pubmed-meshheading:7945238-Stomach Neoplasms,
pubmed-meshheading:7945238-Trypsin,
pubmed-meshheading:7945238-Trypsinogen,
pubmed-meshheading:7945238-Tumor Cells, Cultured
|
|
pubmed:year |
1994
|
|
pubmed:articleTitle |
Identification of one- and two-chain forms of trypsinogen 1 produced by a human gastric adenocarcinoma cell line.
|
|
pubmed:affiliation |
Division of Cell Biology, Kihara Institute for Biological Research, Yokohama City University, Japan.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
