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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
20
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pubmed:dateCreated |
1994-10-27
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pubmed:abstractText |
A severe immunodeficiency involving a complete loss of T lymphocytes and natural killer cells was observed in independent lines of transgenic mice containing > 30 copies of the human CD3E gene (pL12). T-cell- natural killer (NK)- mice could also be generated by using a gene fragment pL12 delta 1 (without exons 4A and 5) coding for the CD3-epsilon transmembrane region and its 55-amino acid nonenzymatic cytoplasmic tail. The abnormally small thymus gland in the homozygous transgenic animals, which was approximately 1% the size of a wild-type thymus, contained only a few (2-4%) prethymocytes with a Thy-1+Pgp-1+IL-2R alpha- CD3-4-8- phenotype. In mice with lower copy numbers of the transgene thymocyte development was blocked at the Thy-1+Pgp-1-IL-2R alpha+CD3-4-8- stage, and normal NK activity was detected. Mice generated with high-copy numbers of a transgene pL12 delta 2 (pL12 delta 1 minus exons 6), coding for a truncated protein from which the CD3-epsilon extracellular domain, its transmembrane region, and most of its cytoplasmic region were absent, contained normal numbers of T lymphocytes and NK cells. These transgene effects suggested that recruitment of signal-transduction molecules by the cytoplasmic tail of this protein played an important role in the abrogation of both lineages. Taken together these observations support the notion that T lymphocytes and NK cells stemmed from a common precursor.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-1372642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-1387664,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-1418374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-1531666,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-1532456,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-1547487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-1555234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-1680927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-2136828,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-2139038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-2188661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-2207317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-2453582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-2583122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-2783950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-2894394,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-3012357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-3267235,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-3497976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-3559207,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-3919309,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-3919312,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-7240748,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-7686857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-8094404,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7937778-8248261
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
91
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pubmed:geneSymbol |
CD3E
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9402-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7937778-Animals,
pubmed-meshheading:7937778-Antibody-Dependent Cell Cytotoxicity,
pubmed-meshheading:7937778-Antigens, CD,
pubmed-meshheading:7937778-Antigens, CD3,
pubmed-meshheading:7937778-Exons,
pubmed-meshheading:7937778-Flow Cytometry,
pubmed-meshheading:7937778-Humans,
pubmed-meshheading:7937778-Immunoblotting,
pubmed-meshheading:7937778-Immunophenotyping,
pubmed-meshheading:7937778-Killer Cells, Natural,
pubmed-meshheading:7937778-Mice,
pubmed-meshheading:7937778-Mice, Inbred C57BL,
pubmed-meshheading:7937778-Mice, Inbred CBA,
pubmed-meshheading:7937778-Mice, Transgenic,
pubmed-meshheading:7937778-Signal Transduction,
pubmed-meshheading:7937778-Spleen,
pubmed-meshheading:7937778-T-Lymphocytes
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pubmed:year |
1994
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pubmed:articleTitle |
A block in both early T lymphocyte and natural killer cell development in transgenic mice with high-copy numbers of the human CD3E gene.
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pubmed:affiliation |
Division of Immunology, Beth Israel Hospital, Harvard Medical School, Boston, MA 02215.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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