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rdf:type |
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lifeskim:mentions |
umls-concept:C0002482,
umls-concept:C0017262,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0061358,
umls-concept:C0085080,
umls-concept:C0086379,
umls-concept:C0167117,
umls-concept:C0185117,
umls-concept:C0205360,
umls-concept:C0247947,
umls-concept:C0378073,
umls-concept:C1167622,
umls-concept:C1521970,
umls-concept:C1879547,
umls-concept:C2911684
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pubmed:issue |
3
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pubmed:dateCreated |
1994-10-28
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pubmed:abstractText |
Glucagon-like peptide-I (GLP-I) is a potent insulinotropic peptide that mediates its actions at pancreatic B-cells via specific receptors. In the present study we stably expressed the rat B-cell GLP-I receptor in CHO cells and studied binding characteristics and receptor activation utilizing the naturally occurring receptor agonist GLP-I(7-36)-amide (GLP-I), the proglucagon-derived GLP-I-related peptide oxyntomodulin, the GLP-I receptor agonist exendin-4, and the specific antagonist exendin(9-39). The potencies to displace [125I]GLP-I from the receptor were GLP-I > exendin-4 > exendin(9-39) > oxyntomodulin, and to displace [125I]exendin-4 GLP-I = exendin-4 > exendin(9-39) > oxyntomodulin. cAMP production was stimulated equally by GLP-I and exendin-4. Oxyntomodulin was less potent to stimulate cAMP generation. Exendin(9-39) blocked the stimulatory action of GLP-I and exendin-4 on cAMP production, but not that of oxyntomodulin. This study shows that GLP-I and exendin-4 are potent agonists at the transfected rat B-cell GLP-I receptor whereas oxyntomodulin is only a weak GLP-I receptor agonist. Furthermore, exendin(9-39) is a potent GLP-I receptor antagonist. This peptide is a valuable tool to further study the physiological actions of GLP-I.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Glucagon,
http://linkedlifedata.com/resource/pubmed/chemical/Glucagon-Like Peptide 1,
http://linkedlifedata.com/resource/pubmed/chemical/Glucagon-Like Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Neurotransmitter Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Oxyntomodulin,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Glucagon,
http://linkedlifedata.com/resource/pubmed/chemical/Venoms,
http://linkedlifedata.com/resource/pubmed/chemical/exenatide,
http://linkedlifedata.com/resource/pubmed/chemical/exendin (9-39),
http://linkedlifedata.com/resource/pubmed/chemical/glucagon-like peptide 1 (7-36)amide,
http://linkedlifedata.com/resource/pubmed/chemical/glucagon-like peptide receptor
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pubmed:status |
MEDLINE
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pubmed:issn |
0196-9781
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
15
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
453-6
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:7937318-Animals,
pubmed-meshheading:7937318-CHO Cells,
pubmed-meshheading:7937318-Cricetinae,
pubmed-meshheading:7937318-Evaluation Studies as Topic,
pubmed-meshheading:7937318-Glucagon,
pubmed-meshheading:7937318-Glucagon-Like Peptide 1,
pubmed-meshheading:7937318-Glucagon-Like Peptides,
pubmed-meshheading:7937318-Lizards,
pubmed-meshheading:7937318-Neurotransmitter Agents,
pubmed-meshheading:7937318-Oxyntomodulin,
pubmed-meshheading:7937318-Peptide Fragments,
pubmed-meshheading:7937318-Peptides,
pubmed-meshheading:7937318-Rats,
pubmed-meshheading:7937318-Receptors, Cell Surface,
pubmed-meshheading:7937318-Receptors, Glucagon,
pubmed-meshheading:7937318-Venoms
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pubmed:year |
1994
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pubmed:articleTitle |
Stable expression of the rat GLP-I receptor in CHO cells: activation and binding characteristics utilizing GLP-I(7-36)-amide, oxyntomodulin, exendin-4, and exendin(9-39).
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pubmed:affiliation |
Department of Medicine, Philipps-University of Marburg, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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