Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
41
|
| pubmed:dateCreated |
1994-11-17
|
| pubmed:abstractText |
Previous analysis of the human interferon-gamma (IFN-gamma promoter indicated that the region of DNA from -251 to -215 (designated here as BE (binding element)) possessed silencer activity, as deletion of this region caused an increase in promoter activity. Based on this finding, we have conducted a series of experiments to characterize BE function and analyze the binding proteins which interact with this region. Transient transfection assays in the Jurkat T cell line revealed that the BE region possesses silencer activity, which is orientation-dependent when reinserted 5' to the IFN-gamma core promoter. However, when the BE region was inserted in front of a heterologous promoter (thymidine kinase (TK)), a mild enhancer activity was observed. Utilizing the electrophoretic mobility shift assay, we have identified two major DNA-protein complexes (designated as S and E complexes) which interact with this region. Mutational analysis indicated that the silencer activity observed with the IFN-gamma promoter correlated with the S complex and the enhancer activity correlated with the E complex. Preliminary characterization of these two DNA-protein complexes has demonstrated the presence of multiple proteins in each complex. We have found that the S protein complex has a recognition sequence similar to the nuclear factor AP2, and we have identified the nuclear factor Yin-Yang 1 (YY1) as one of the proteins in the E complex.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Erythroid-Specific DNA-Binding...,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Macromolecular Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/YY1 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/YY1 protein, human
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
14
|
| pubmed:volume |
269
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
25728-34
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:7929277-Base Sequence,
pubmed-meshheading:7929277-Binding Sites,
pubmed-meshheading:7929277-CD4-Positive T-Lymphocytes,
pubmed-meshheading:7929277-Cell Line,
pubmed-meshheading:7929277-DNA Mutational Analysis,
pubmed-meshheading:7929277-DNA-Binding Proteins,
pubmed-meshheading:7929277-Erythroid-Specific DNA-Binding Factors,
pubmed-meshheading:7929277-Gene Expression Regulation,
pubmed-meshheading:7929277-Genes, Reporter,
pubmed-meshheading:7929277-Humans,
pubmed-meshheading:7929277-Interferon-gamma,
pubmed-meshheading:7929277-Macromolecular Substances,
pubmed-meshheading:7929277-Molecular Sequence Data,
pubmed-meshheading:7929277-Nuclear Proteins,
pubmed-meshheading:7929277-Promoter Regions, Genetic,
pubmed-meshheading:7929277-Protein Binding,
pubmed-meshheading:7929277-Transcription Factors,
pubmed-meshheading:7929277-Transfection,
pubmed-meshheading:7929277-YY1 Transcription Factor
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Characterization of a silencer regulatory element in the human interferon-gamma promoter.
|
| pubmed:affiliation |
Laboratory of Experimental Immunology, Program Resources Inc./DynCorp, NCI-FCRDC, Frederick, Maryland 21702-1201.
|
| pubmed:publicationType |
Journal Article
|