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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0017262,
umls-concept:C0037083,
umls-concept:C0449432,
umls-concept:C1179435,
umls-concept:C1524073,
umls-concept:C1548799,
umls-concept:C1705248,
umls-concept:C1709059,
umls-concept:C1710082,
umls-concept:C1879547
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pubmed:issue |
41
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pubmed:dateCreated |
1994-11-17
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pubmed:abstractText |
To investigate cell cycle-dependent expression of interferon (IFN)-induced genes and signaling pathways, we examined the modulation of IFN-inducible gene expression and p91 activation, and JAK-1 and Tyk-2 kinases' activation in human Burkitt's lymphoma Daudi cells which were synchronized at different points in the cell cycle progression. We observed that each one of 100-, 67-, and 46-kDa 2'-5'-oligoadenylate synthetase (2-5(A)-synthetase) proteins had a unique expression pattern in different phases of the cell cycle which was different from the expression of 2-5(A)-synthetase proteins in exponentially growing Daudi cells. The cell cycle-dependent changes in the overall levels of expression of 2-5(A)-synthetase proteins correlated well with the induction of expression of 2-5(A)-synthetase mRNA as well as the appearance of IFN stimulatory gene factor-3 and expression and activation of p91 in cells released from the G1/S block in the absence of exogenous IFN-alpha. In addition, cells from the G1/S phase exhibited increased tyrosine phosphorylation of JAK-1 and Tyk-2 kinases and p91 by IFN-alpha as compared with asynchronized cells. Enhanced stimulation of activation of JAK-1, Tyk-2, and p91 by IFN-alpha was progressively reduced from G1/S to G2/M to pass G2/M. These findings demonstrate that the levels of constitutive and inducible expression of IFN-alpha-inducible genes, activation of p91, and tyrosine phosphorylation of JAK-1 and Tyk-2 kinases are differentially influenced by the state of the cell cycle.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2',5'-Oligoadenylate Synthetase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/JAK1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Janus Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/STAT1 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/STAT1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/TYK2 Kinase,
http://linkedlifedata.com/resource/pubmed/chemical/TYK2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
269
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
25437-41
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:7929242-2',5'-Oligoadenylate Synthetase,
pubmed-meshheading:7929242-Base Sequence,
pubmed-meshheading:7929242-Burkitt Lymphoma,
pubmed-meshheading:7929242-Cell Cycle,
pubmed-meshheading:7929242-DNA-Binding Proteins,
pubmed-meshheading:7929242-Enzyme Activation,
pubmed-meshheading:7929242-G1 Phase,
pubmed-meshheading:7929242-Gene Expression Regulation,
pubmed-meshheading:7929242-Humans,
pubmed-meshheading:7929242-Interferon-alpha,
pubmed-meshheading:7929242-Janus Kinase 1,
pubmed-meshheading:7929242-Molecular Sequence Data,
pubmed-meshheading:7929242-Protein-Tyrosine Kinases,
pubmed-meshheading:7929242-Proteins,
pubmed-meshheading:7929242-S Phase,
pubmed-meshheading:7929242-STAT1 Transcription Factor,
pubmed-meshheading:7929242-Signal Transduction,
pubmed-meshheading:7929242-TYK2 Kinase,
pubmed-meshheading:7929242-Trans-Activators,
pubmed-meshheading:7929242-Tumor Cells, Cultured
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pubmed:year |
1994
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pubmed:articleTitle |
Cell cycle-dependent modulation of alpha-interferon-inducible gene expression and activation of signaling components in Daudi cells.
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pubmed:affiliation |
Department of Medicine, Pennsylvania State University College of Medicine, Hershey 17033.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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