Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
1994-10-12
|
| pubmed:abstractText |
D-Alanine was administered orally to mutant mice lacking D-amino acid oxidase (EC 1.4.3.3). The mice had free access to drinking water containing 0.5% D- or L-alanine or 0.1% D-alanine for 2 weeks. The mice were then killed, and levels of the D- and L-enantiomers of free alanine, serine, proline, glutamate, and aspartate were determined in serum, liver, kidney, cerebrum, and cerebellum tissues. D-Alanine content increased by 60-fold (liver) to 110-fold (serum, brain), although the L-alanine level did not change. The increase of serum and brain D-alanine concentrations in animals fed 0.5% D-alanine was approximately five times more than that in animals fed 0.1% D-alanine, ie, the increase was roughly D-alanine dose-dependent in these tissues. The increase due to 0.5% D-alanine administration was reduced by 50% 17 hours after administration of D-alanine was stopped. Administration-induced increases in D-alanine levels in the cerebrum and cerebellum were not less than those in the serum, suggesting that D-alanine passed the blood-brain barrier quite freely. In the liver but not in other tissues, there were slight increases in D-serine and D-proline levels after administration of D-alanine. Administration of D-alanine produced no alterations in free glutamate and aspartate levels. No D-enantiomers of alanine, serine, proline, glutamate, or aspartate were detected in the liver and kidney tissue proteins of any animals, even in the mutant mice that received 0.5% D-alanine.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Sep
|
| pubmed:issn |
0026-0495
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
43
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1153-7
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:7916120-Alanine,
pubmed-meshheading:7916120-Amino Acids,
pubmed-meshheading:7916120-Animals,
pubmed-meshheading:7916120-Brain,
pubmed-meshheading:7916120-D-Amino-Acid Oxidase,
pubmed-meshheading:7916120-Drinking,
pubmed-meshheading:7916120-Kidney,
pubmed-meshheading:7916120-Liver,
pubmed-meshheading:7916120-Mice,
pubmed-meshheading:7916120-Mice, Mutant Strains,
pubmed-meshheading:7916120-Stereoisomerism,
pubmed-meshheading:7916120-Water
|
| pubmed:year |
1994
|
| pubmed:articleTitle |
Amino acid levels in D-alanine-administered mutant mice lacking D-amino acid oxidase.
|
| pubmed:affiliation |
Department of Life Science, Himeji Institute of Technology, Hyogo, Japan.
|
| pubmed:publicationType |
Journal Article
|