Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1994-6-16
pubmed:abstractText
The toxin fraction (FTX) and peptide omega-Aga-IVA from the venom of the funnel-web spider Agelenopsis aperta, as well as a synthetic analogue of FTX, specifically block the P-type voltage-dependent Ca2+ channel (VDCC). The effects of these toxins on synaptic transmission were studied in the neuromuscular synapses of the crayfish opener muscle, which has a single excitatory and a single inhibitory motoneuron. FTX selectively and reversibly blocked excitatory and inhibitory postsynaptic currents and potentials in a dose-dependent manner. FTX had no effect on (i) resting and postsynaptic membrane conductance, (ii) postsynaptic L-type VDCC, and (iii) both glutamate- and gamma-aminobutyric acid-induced postsynaptic responses. Mean amplitude and frequency of miniature postsynaptic potentials were unchanged by FTX. The postsynaptic VDCC was inhibited by nifedipine, a selective dihydropyridine antagonist of L-type VDCC, whereas synaptic transmission was unaffected. Transmission was also undisturbed by omega-conotoxin, suggesting that N-type VDCCs are not involved. The peptide omega-Aga-IVA blocked excitatory and inhibitory transmission without affecting postsynaptic VDCC. Synaptic transmission was also blocked by synthetic FTX. We conclude that presynaptic P-type VDCCs are involved in both evoked excitatory and inhibitory transmitter release in crayfish neuromuscular synapses.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-1310319, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-1311418, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-13175198, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-1319261, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-1348859, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-1357749, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-1683761, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-1684129, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-2010819, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-2165923, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-2428039, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-2430302, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-2432656, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-2433275, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-2438698, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-2447647, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-2450999, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-2469160, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-2478694, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-2537980, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-2547195, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-2560643, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-2575161, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-2577439, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-2664080, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-4071055, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-5357245, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-6320053, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-6608056, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-7264992, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-7683716, http://linkedlifedata.com/resource/pubmed/commentcorrection/7910404-874908
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channel Blockers, http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels, http://linkedlifedata.com/resource/pubmed/chemical/FTX, spider toxin, http://linkedlifedata.com/resource/pubmed/chemical/Glutamates, http://linkedlifedata.com/resource/pubmed/chemical/Glutamic Acid, http://linkedlifedata.com/resource/pubmed/chemical/Neurotoxins, http://linkedlifedata.com/resource/pubmed/chemical/Nifedipine, http://linkedlifedata.com/resource/pubmed/chemical/Polyamines, http://linkedlifedata.com/resource/pubmed/chemical/Spider Venoms, http://linkedlifedata.com/resource/pubmed/chemical/Tetraethylammonium, http://linkedlifedata.com/resource/pubmed/chemical/Tetraethylammonium Compounds, http://linkedlifedata.com/resource/pubmed/chemical/gamma-Aminobutyric Acid, http://linkedlifedata.com/resource/pubmed/chemical/omega-Agatoxin IVA
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
91
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4224-8
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:7910404-Animals, pubmed-meshheading:7910404-Astacoidea, pubmed-meshheading:7910404-Calcium Channel Blockers, pubmed-meshheading:7910404-Calcium Channels, pubmed-meshheading:7910404-Evoked Potentials, pubmed-meshheading:7910404-Glutamates, pubmed-meshheading:7910404-Glutamic Acid, pubmed-meshheading:7910404-Membrane Potentials, pubmed-meshheading:7910404-Motor Neurons, pubmed-meshheading:7910404-Muscles, pubmed-meshheading:7910404-Neuromuscular Junction, pubmed-meshheading:7910404-Neurotoxins, pubmed-meshheading:7910404-Nifedipine, pubmed-meshheading:7910404-Polyamines, pubmed-meshheading:7910404-Spider Venoms, pubmed-meshheading:7910404-Synaptic Transmission, pubmed-meshheading:7910404-Tetraethylammonium, pubmed-meshheading:7910404-Tetraethylammonium Compounds, pubmed-meshheading:7910404-gamma-Aminobutyric Acid, pubmed-meshheading:7910404-omega-Agatoxin IVA
pubmed:year
1994
pubmed:articleTitle
P-type Ca2+ channels mediate excitatory and inhibitory synaptic transmitter release in crayfish muscle.
pubmed:affiliation
Instituto Cajal Consejo Superior de Investigaciones Cientificas, Madrid, Spain.
More...