|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
1994-4-25
|
|
pubmed:abstractText |
A full-length cDNA clone of the human dopamine D3 receptor was obtained by the polymerase chain reaction (PCR) using reverse-transcribed RNA from human brain as the template. The cDNA was inserted into an expression vector which was then stably transfected into either Chinese hamster ovary (CHO), SK-N-MC human epithelioma or mouse CCL1.3 fibroblast cell lines. Post-transfection, the Bmax for D3 receptor expression was 1.9, 1.1 and 0.4 pmol/mg protein in the CHO-K1, SK-N-MC and CCL1.3 cell lines, respectively. The D3 receptor expressed in CHO-K1 and CCL1.3 cells exhibited similar radioligand binding profiles, especially for the D3-selective compound, 7-hydroxy-2-(di-n-propylamino)tetralin (7-OH-DPAT). Radioligand-binding competition curves of presumed D3 agonists were shifted to the right by the addition of guanine nucleotides and Na+ to the assay buffer. Presumed D3-receptor agonists had no effect on cAMP accumulation in any of the D3-transfected cell lines although cAMP accumulation was inhibited by dopamine D2 receptor activation in D2-transfected CHO and CCL1.3 cells and by activation of the exogenously expressed neuropeptide Y receptor in SK-N-MC cells. Also, D3 receptor activation neither potentiated ATP-stimulated arachidonic acid release from CHO cells nor stimulated inositol phosphate production in CCL1.3-cells although both of these responses were elicited by D2 agonists in D2-transfected cells. We conclude that the signalling properties of the D3 receptor differ from those of its closest homolog, the D2 receptor.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/7-hydroxy-2-N,N-dipropylaminotetrali...,
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/DRD3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Dopamine Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Dopamine Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Drd3 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Dopamine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Dopamine D2,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Dopamine D3,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrahydronaphthalenes
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Jan
|
|
pubmed:issn |
0014-2999
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
1
|
|
pubmed:volume |
266
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
79-85
|
|
pubmed:dateRevised |
2005-11-17
|
|
pubmed:meshHeading |
pubmed-meshheading:7907989-Animals,
pubmed-meshheading:7907989-Arachidonic Acid,
pubmed-meshheading:7907989-Binding, Competitive,
pubmed-meshheading:7907989-CHO Cells,
pubmed-meshheading:7907989-Cell Line,
pubmed-meshheading:7907989-Cloning, Molecular,
pubmed-meshheading:7907989-Cricetinae,
pubmed-meshheading:7907989-Cricetulus,
pubmed-meshheading:7907989-Cyclic AMP,
pubmed-meshheading:7907989-Dopamine Agents,
pubmed-meshheading:7907989-Dopamine Antagonists,
pubmed-meshheading:7907989-Humans,
pubmed-meshheading:7907989-Inositol Phosphates,
pubmed-meshheading:7907989-Mice,
pubmed-meshheading:7907989-Radioligand Assay,
pubmed-meshheading:7907989-Receptors, Dopamine,
pubmed-meshheading:7907989-Receptors, Dopamine D2,
pubmed-meshheading:7907989-Receptors, Dopamine D3,
pubmed-meshheading:7907989-Tetrahydronaphthalenes,
pubmed-meshheading:7907989-Transfection
|
|
pubmed:year |
1994
|
|
pubmed:articleTitle |
Characterization of the human dopamine D3 receptor expressed in transfected cell lines.
|
|
pubmed:affiliation |
Parke-Davis, Department of Pharmacology, Ann Arbor, MI 48106.
|
|
pubmed:publicationType |
Journal Article
|
