Linked Life Data

7903083

Source:http://linkedlifedata.com/resource/pubmed/id/7903083

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1994-1-19
pubmed:abstractText
Eleven multiply resistant Enterobacter cloacae isolates were obtained from eight preterm neonates in a neonatal intensive care unit (NICU) of one hospital in Osnabrück, together with one sensitive strain from another infant. The presence of similar antibiograms and biotypes in 11 isolates prompted further characterization of the isolates by pulsed-field gel electrophoresis (PFGE) of NotI generated genomic restriction fragments. For assessment of the discriminatory power of this typing method 50 non-related strains were included in the study. Non-related strains demonstrated a marked variation in restriction fragment patterns and were clearly discriminated one from another. In contrast, 11 of 12 isolates from the NICU exhibited identical restriction profiles, indicating a cluster associated with nosocomial infection. Since the discriminatory power of PFGE is high, reproducibility is good, and all reagents and equipment for DNA fingerprinting are commercially available, this technique is proposed as a useful tool in the microbiology laboratory for investigating the epidemiology of E. cloacae strains.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0195-6701
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
109-16
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1993
pubmed:articleTitle
Molecular typing of Enterobacter cloacae by pulsed-field gel electrophoresis of genomic restriction fragments.
pubmed:affiliation
Staatliches Medizinal-Untersuchungsamt Osnabrück, Germany.
pubmed:publicationType
Journal Article, Comparative Study