Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3 Pt 2
|
| pubmed:dateCreated |
1995-4-27
|
| pubmed:abstractText |
The actions of angiotensin II (ANG II) were examined in the spontaneously active cells isolated from the rabbit sinoatrial node, using the nystatin-permeabilized, whole cell, patch-clamp method. At 30 nM, ANG II significantly lowered the spontaneous firing rate of the action potentials from 212 +/- 21 to 172 +/- 32 beats/min, with a concomitant reduction in the action potential amplitude. The voltage-clamp experiments showed that ANG II inhibited the L-type Ca2+ current (ICa) with a dissociation constant (Kd) of approximately 4 nM and a maximal inhibition of 30%. The inhibition was blocked by an AT1-receptor antagonist CV11974. Acetylcholine (ACh) at 10 microM reduced the ICa by 42 +/- 12%, and ANG II did not cause any further inhibition in the presence of ACh. At 100 nM, ANG II reduced the ICa by only 12% in the presence of 2 microM isoproterenol, and a similar inhibition was observed with 0.1 microM ACh. ANG II did not affect the dibutyryl adenosine 3',5'-cyclic monophosphate-stimulated ICa. Protein kinase C activator 12-O-tetra-decanoylphorbol-13-acetate did not mimic ANG II in the effects on ICa, and preincubation of the cells with calphostin C, a protein kinase C inhibitor, did not attenuate the ANG II effect. ANG II exerts a negative chronotropic effect in the pacemaker cells as its direct action through a pathway involving adenosine 3',5'-cyclic monophosphate-dependent protein kinase.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetylcholine,
http://linkedlifedata.com/resource/pubmed/chemical/Angiotensin II,
http://linkedlifedata.com/resource/pubmed/chemical/Angiotensin Receptor Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Benzimidazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP-Dependent Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Tetrazoles,
http://linkedlifedata.com/resource/pubmed/chemical/candesartan
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0002-9513
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
268
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
H1053-60
|
| pubmed:dateRevised |
2010-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:7900859-Acetylcholine,
pubmed-meshheading:7900859-Action Potentials,
pubmed-meshheading:7900859-Angiotensin II,
pubmed-meshheading:7900859-Angiotensin Receptor Antagonists,
pubmed-meshheading:7900859-Animals,
pubmed-meshheading:7900859-Benzimidazoles,
pubmed-meshheading:7900859-Calcium,
pubmed-meshheading:7900859-Cyclic AMP-Dependent Protein Kinases,
pubmed-meshheading:7900859-Heart Rate,
pubmed-meshheading:7900859-Intracellular Fluid,
pubmed-meshheading:7900859-Ion Transport,
pubmed-meshheading:7900859-Kinetics,
pubmed-meshheading:7900859-Membrane Potentials,
pubmed-meshheading:7900859-Myocardial Contraction,
pubmed-meshheading:7900859-Rabbits,
pubmed-meshheading:7900859-Renin-Angiotensin System,
pubmed-meshheading:7900859-Sinoatrial Node,
pubmed-meshheading:7900859-Tetrazoles
|
| pubmed:year |
1995
|
| pubmed:articleTitle |
Angiotensin II inhibition of L-type Ca2+ current in sinoatrial node cells of rabbits.
|
| pubmed:affiliation |
Department of Laboratory Medicine, Kyoto Prefectural University of Medicine, Japan.
|
| pubmed:publicationType |
Journal Article,
In Vitro
|