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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3 Pt 2
|
| pubmed:dateCreated |
1995-4-27
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| pubmed:abstractText |
Angiotensin II (ANG II) induces vascular smooth muscle contraction and functions as a growth factor stimulating vascular smooth muscle cell (VSMC) hypertrophy. We wondered whether ANG II might induce expression of alpha 1-adrenergic receptors (ARs) in cultured VSMCs, which would then potentially accentuate the effects of catecholamines in the cells. Rat VSMCs were preincubated with phenoxybenzamine to inactivate alpha 1-ARs; the cells were then treated with ANG II for 24 h. ANG II-treated cells expressed an increased number of alpha 1-ARs compared with controls, suggesting that ANG II increased the rate of alpha 1-AR synthesis. ANG II markedly induced accumulation of alpha 1A/D- and alpha 1B-AR mRNAs in a concentration- and time-dependent manner. This effect of ANG II was blocked by the specific ANG II-receptor agonist [Sar1-Ile8]ANG II. ANG II-induced accumulation of alpha 1A/D-AR mRNAs was completely abolished by transcriptional inhibitor actinomycin D. ANG II markedly increased the transcriptional rate of the alpha 1A/D-AR gene without changing stability of alpha 1A/D-AR mRNAs. Protein kinase C (PKC) activator 4 beta-phorbol 12-myristate 13-acetate also induced accumulation of alpha 1A/D-AR mRNAs, and PKC inhibitor H-7 completely blocked ANG II-induced accumulation of the alpha 1A/D-AR mRNAs. Neither the biologically inactive ANG II analogue des-Phe8-ANG II nor the calcium ionophores A-23187 and BAY K-8466 induced alpha 1A/D-AR mRNAs. Finally, ANG II preincubation increased alpha 1-AR agonist phenylephrine-stimulated expression of the c-fos gene.(ABSTRACT TRUNCATED AT 250 WORDS)
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-Sarcosine-8-Isoleucine...,
http://linkedlifedata.com/resource/pubmed/chemical/Angiotensin II,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Phenoxybenzamine,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Adrenergic, alpha-1
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| pubmed:status |
MEDLINE
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| pubmed:month |
Mar
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| pubmed:issn |
0002-9513
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
268
|
| pubmed:geneSymbol |
c-fos
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
H1006-14
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| pubmed:dateRevised |
2007-11-15
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| pubmed:meshHeading |
pubmed-meshheading:7900855-1-Sarcosine-8-Isoleucine Angiotensin II,
pubmed-meshheading:7900855-Amino Acid Sequence,
pubmed-meshheading:7900855-Angiotensin II,
pubmed-meshheading:7900855-Animals,
pubmed-meshheading:7900855-Base Sequence,
pubmed-meshheading:7900855-Cells, Cultured,
pubmed-meshheading:7900855-DNA Primers,
pubmed-meshheading:7900855-Gene Expression,
pubmed-meshheading:7900855-Genes, fos,
pubmed-meshheading:7900855-Humans,
pubmed-meshheading:7900855-Molecular Sequence Data,
pubmed-meshheading:7900855-Muscle, Smooth, Vascular,
pubmed-meshheading:7900855-Phenoxybenzamine,
pubmed-meshheading:7900855-Protein Kinase C,
pubmed-meshheading:7900855-RNA, Messenger,
pubmed-meshheading:7900855-Rats,
pubmed-meshheading:7900855-Receptors, Adrenergic, alpha-1,
pubmed-meshheading:7900855-Transcription, Genetic
|
| pubmed:year |
1995
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| pubmed:articleTitle |
Angiotensin II induces transcription and expression of alpha 1-adrenergic receptors in vascular smooth muscle cells.
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| pubmed:affiliation |
Department of Medicine, Stanford University School of Medicine, Palo Alto, California.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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