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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
1995-4-21
|
| pubmed:abstractText |
Hantaan virus often causes a fatal human disease, hemorrhagic fever with renal syndrome (HFRS). An assay for strand-specific detection and quantitation of Hantaan virus RNA was developed based on the polymerase chain reaction (PCR). A protocol for the efficient detection of both genomic RNA and its transcript is presented, in which a reverse transcriptase reaction (RTR) followed by PCR with two common primers was used to distinguish between positive- and negative-stranded RNA. Using this method, the growth characteristics of Hantaan virus, strain 76-118, were studied in Vero E6 cells. Positive-stranded RNA was detected on the next day after infection, and the amount increased remarkably beginning from 3 days after infection. The negative-stranded RNA (genomic), was detected at 2 days after infection which predominated over the transcript RNA. Three days after infection, the amount of viral RNA attained 80% of the maximum amount which was detected by 6 days after infection, while, 4 days after infection the amount of the positive-stranded RNA became over 80% of the maximum amount of 6 days after infection and reached the amount of viral RNA. Both RNAs reached plateaus at 6 days after infection and after that the amounts of synthesized viral RNA and its transcripts were constant.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0385-5600
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
38
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
905-8
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading | |
| pubmed:year |
1994
|
| pubmed:articleTitle |
Strand-specific detection of Hantaan virus RNA sequences by in vitro DNA amplification.
|
| pubmed:affiliation |
Department of Virology, Osaka University, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|