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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1995-4-19
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pubmed:abstractText |
1. The secretory responses to calcitonin gene-related peptide (CGRP) receptor agonists have been characterized in two human adenocarcinoma cell lines, namely HCA-7 and Colony-29 (Col-29) epithelia. These cells form polarized epithelial layers when grown on permeable supports and allow changes in electrogenic ion transport in response to agonists to be monitored continuously. 2. alpha-CGRP (rat and human sequences), rat beta-CGRP and human [Tyr0]CGRP applied to the basolateral surface were found to be full agonists, causing prolonged increases in short-circuit current. Concentration-response curves exhibited EC50 values of 0.6-1.5 nM in HCA-7 cells. The same agonists were less effective in Col-29 epithelia, the EC50 values ranging from 1 to 10 nM in these cells. [Cys(ACM)2,7]CGRP was effective in both cell lines and was more potent in HCA-7 cells. 3. CGRP receptors were preferentially located on the basolateral surface in both cell types. Addition of r alpha-CGRP to the apical domain produced significantly smaller secretory responses (8.1% in HCA-7 and 29.2% in Col-29) compared with those produced following basolateral application (100%). 4. In both cell lines r alpha-CGRP-elevated short-circuit current was inhibited by the loop diuretic piretanide (200 microM) and by somatostatin (100 nM). Pretreating epithelia with the cyclo-oxygenase inhibitor, piroxicam (5 microM) had no significant effect upon CGRP responses in either cell line. 5. Rat alpha-CGRP (0.2 nM) responses in HCA-7 epithelia were inhibited by the C-terminal fragment CGRP(8-37) (1 microM). Pretreatment of Col-29 cells with CGRP(8-37) did not, however, alter the size or profile of responses to r alpha-CGRP (1 nM).6. We conclude that high-affinity CGRP receptors exist on the basolateral surface of both cell lines,however they differ in their sensitivity to CGRP(8-37) and agonist orders of potency. Thus different CGRP receptor subtypes appear to predominate in these two epithelial cell types.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/7889279-1280118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7889279-1313730,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7889279-1319450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7889279-1706667,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7889279-2464148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7889279-2464161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7889279-2536720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7889279-2553933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7889279-2787038,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7889279-2790388,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7889279-2839796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7889279-3038239,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7889279-4016751,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7889279-8132005,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7889279-8381072,
http://linkedlifedata.com/resource/pubmed/commentcorrection/7889279-8383565
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0007-1188
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
113
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1243-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:7889279-Animals,
pubmed-meshheading:7889279-Calcitonin Gene-Related Peptide,
pubmed-meshheading:7889279-Cell Line,
pubmed-meshheading:7889279-Diuretics,
pubmed-meshheading:7889279-Electrophysiology,
pubmed-meshheading:7889279-Epithelial Cells,
pubmed-meshheading:7889279-Epithelium,
pubmed-meshheading:7889279-Gastric Mucosa,
pubmed-meshheading:7889279-Humans,
pubmed-meshheading:7889279-Intestinal Mucosa,
pubmed-meshheading:7889279-Piroxicam,
pubmed-meshheading:7889279-Rats,
pubmed-meshheading:7889279-Receptors, Calcitonin Gene-Related Peptide,
pubmed-meshheading:7889279-Somatostatin,
pubmed-meshheading:7889279-Sulfonamides,
pubmed-meshheading:7889279-Tumor Cells, Cultured
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pubmed:year |
1994
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pubmed:articleTitle |
Calcitonin gene-related peptide receptors in human gastrointestinal epithelia.
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pubmed:affiliation |
Department of Pharmacology, Royal College of Surgeons of England, London.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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